首页 > Biovendor > Biovendor/Prohibitin Human E. coli/RD172239100/0.1 mg

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Biovendor/Prohibitin Human E. coli/RD172239100/0.1 mg

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RD172239100

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p.chemkody:after{ content:""; display:block; overflow:hidden; clear:both; height:0; } p.chemkody span:nth-of-type(7n+1) { clear: left; } p.chemkody span { float: left; min-width: 90px; margin: 0 20px 0 0; padding: 0 0 10px; color: #6e6e6e; font-family: Courier New,monospace; white-space: nowrap; }Regulatorystatus:RUOType:RecombinantSource:E.coliOthernames:PHBSpecies:HumanTypeRecombinantDescriptionTotal282 AA.MW:31 kDa(calculated).UniProtKBacc.no.P35232(Met1-Gln272).N-terminalHis-tag(10 extraAA).ProteinidentityconfirmedbyLC-MS/MS.AminoAcidSequenceMKHHHHHHASMAAKVFESIGKFGLALAVAGGVVNSALYNVDAGHRAVIFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCRSRPRNVPVITGSKDLQNVNITLRILFRPVASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKAEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLSRSRNITYLPAGQSVLLQLPQSourceE.coliPurityPurityasdeterminedbydensitometricimageanalysis:≥90 %SDS-PAGEGel14%SDS-PAGEseparationofHumanProhibitin:1. M.W.Marker –14,21,31,45,66,97 kDa2.reducedandboiledsample,2.5μg/lane3.non-reducedandnon-boiledsample,2.5μg/laneEndotoxin<1.0 EU/ugFormulationFiltered(0,4 μm)andlyophilizedin0.5 mg/mLin0.02 Mtrisbuffer,0.05 MNaCl,pH 8.6ReconstitutionAdddeionizedwatertoprepareaworkingstocksolutionofapproximately0.25 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.ApplicationsWesternblotting,ELISAShippingAtambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.Storage/ExpirationStorethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.QualityControlTestBCAtodeterminequantityoftheprotein.SDSPAGEtodeterminepurityoftheprotein.LALtodeterminequantityofendotoxin.NoteThisproductisintendedforresearchuseonly

miREIA是一种基于免疫测定的新型miRNA定量方法，涉及从患者样品中分离出的miRNA与互补的生物素化DNA寡核苷酸探针杂交。然后通过固定有完美匹配的DNA / miRNA杂交体的微量滴定板固定的单克隆抗体捕获DNA / RNA杂交体。下一步是复制标准ELISA方案。

该测定可在常规免疫测定设备上运行，与标准临床工作流程兼容，不需要扩增步骤，并且包括miRNA分析在内的结果可在不到三个小时的时间内获得。