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Biovendor/Interleukin-1β Murine E. coli/RBG20001B100/100 μg

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RBG20001B100

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p.chemkody:after{ content:""; display:block; overflow:hidden; clear:both; height:0; } p.chemkody span:nth-of-type(7n+1) { clear: left; } p.chemkody span { float: left; min-width: 90px; margin: 0 20px 0 0; padding: 0 0 10px; color: #6e6e6e; font-family: Courier New,monospace; white-space: nowrap; }Regulatorystatus:RUOType:RecombinantSource:E.coliOthernames:Catabolin,Lymphocyte-activatingfactor(LAF),EndogenousPyrogen(EP),LeukocyteEndogenousMediator(LEM),MononuclearCellFactor(MCF),Interleukin-1ßSpecies:MurineTypeRecombinantDescriptionIL-1βisaproinflammatorycytokineproducedinavarietyofcells,includingmonocytes,tissuemacrophages,keratinocytes,andotherepithelialcells.BothIL-1αandIL-1βbindtothesamereceptorandhavesimilar,ifnotidentical,BIOLOGicalproperties.ThesecytokineshaveabroadrangeofactivitiesincludingthestimulationofthymocyteproliferationbyinducingIL-2 release,B-cellmaturationandproliferation,mitogenicFGF-likeactivity,andthereleaseofprostaglandinandCollagenasefromsynovialcells.However,whereasIL-1βisasecretedcytokine,IL-1αispredominantlyacell-associatedcytokine.RecombinantMurineIL-1βisa17.5 kDaproteincontaining153 aminoacidresidues.AminoAcidSequenceMVPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSSSourceE.coliPurity98%BiologicalActivityTheED50asdeterminedbythedose-dependentstimulationofmurineD10Scellsis0.002 ng/ml,correspondingtoaspecificactivityof5×108units/mg.EndotoxinEndotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).ReconstitutionCentrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.Storage/Expiration –20°C

miREIA是一种基于免疫测定的新型miRNA定量方法，涉及从患者样品中分离出的miRNA与互补的生物素化DNA寡核苷酸探针杂交。然后通过固定有完美匹配的DNA / miRNA杂交体的微量滴定板固定的单克隆抗体捕获DNA / RNA杂交体。下一步是复制标准ELISA方案。

该测定可在常规免疫测定设备上运行，与标准临床工作流程兼容，不需要扩增步骤，并且包括miRNA分析在内的结果可在不到三个小时的时间内获得。