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Biovendor/Clusterin Rat ELISA/RD391034200CS/96 wells (1 kit)
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产品说明
p.chemkody:after{ content:""; display:block; overflow:hidden; clear:both; height:0; } p.chemkody span:nth-of-type(7n+1) { clear: left; } p.chemkody span { float: left; min-width: 90px; margin: 0 20px 0 0; padding: 0 0 10px; color: #6e6e6e; font-family: Courier New,monospace; white-space: nowrap; } Regulatory status:RUO Type:Sandwich ELISA, Biotin-labelled antibody Other names:Apolipoprotein J, Apo J Species:Rat Type Sandwich ELISA, Biotin-labelled antibody Applications Serum, Urine Sample Requirements 5 µl/well Storage/Expiration Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box). Calibration Curve Calibration Range 4–128 ng/ml Limit of Detection Limit of detection(LOD) (defined as concentration of analyte giving absorbance higher than mean absorbance of blank* plus three standard deviations of the absorbance of blank: Ablank + 3×SDblank) is 0.7 ng/ml. Intra-assay (Within-Run) n = 8; CV = 4.4% Inter-assay (Run-to-Run) n = 8; CV = 5.9% Spiking Recovery 97,40% Dilutation Linearity 100,10% Crossreactivity bovine Non-detectable cat Non-detectable dog Non-detectable goat Non-detectable hamster Non-detectable horse Non-detectable human Non-detectable monkey Non-detectable pig Non-detectable rabbit Non-detectable sheep Non-detectable chicken Not tested rat Yes mouse Yes (recommended dilution 1:500)

miREIA是一种基于免疫测定的新型miRNA定量方法,涉及从患者样品中分离出的miRNA与互补的生物素化DNA寡核苷酸探针杂交。然后通过固定有完美匹配的DNA / miRNA杂交体的微量滴定板固定的单克隆抗体捕获DNA / RNA杂交体。下一步是复制标准ELISA方案。

该测定可在常规免疫测定设备上运行,与标准临床工作流程兼容,不需要扩增步骤,并且包括miRNA分析在内的结果可在不到三个小时的时间内获得。

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