OVERVIEW: Synonyms Monoclonal HIV1 Nef antibody, Anti-HIV1 Nef antibody, C terminal core protein antibody, Negative factor antibody, ABIN antibody, HIV-1 Nef antibody, ABIN1 antibody, HIV nef 1 antibody, HIV nef -1 antibody, p27 antibody, HIV nef 1, VAN antibody , 3'ORF antibody, Nef antibody, HIV nef -1, F protein antibody, HIV1 nef Specificity HIV-1 nef Cross Reactivity To be determined by end-user Applications ELISA, WB Immunogen HIV1 Nef antibody was raised in mouse using full length recombinant nef (HIV-1) as the immunogen. SPECIFICATIONS: Host Mouse Clone 2H12 Isotype IgG1 Purity > 95% pure Form & Buffer Supplied lyophilized from PBS buffer, with no preservatives added, reconstitute in sterile distilled water. STORAGE & SAFETY: Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -70 deg C for long term storage. Avoid repeated freeze/thaw cycles. Usage Assay Information: Usage Recommendations ELISA: 1:1,000, WB: 1:1,000 GENERAL INFORMATION: Biological Significance Nef (Negative Regulatory Factor) is a protein expressed by primate lentiviruses. These include human immunodeficiency viruses (HIV-1 and HIV-2) and simian immunodeficiency virus (SIV). Nef is one of many pathogen-expressed proteins, known as virulence factors, which function to manipulate the host's cellular machinery and thus allow infection, survival or replication of the pathogen. Nef stands for "Negative Factor".