OVERVIEW: Synonyms Monoclonal Fascin antibody, Anti-Fascin antibody Specificity Human Cross Reactivity To be determined by end-user Applications IHC, WB Immunogen Fascin antibody was raised in mouse using prokaryotic recombinant protein corresponding to the C-terminal region of the fascin molecule as the immunogen. SPECIFICATIONS: Host Mouse Clone IM20 Isotype IgG1 kappa Molecular Weight 55-58 kDa (predicted detection band MW) Form & Buffer Lyophilized tissue culture supernatant containing 15mM NaN3. Reconstitute with 1 ml of sterile distilled water. STORAGE & SAFETY: Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling. Usage Assay Information: Usage Recommendations IHC: 1:200-1:400, WB: 1:100-1:200 GENERAL INFORMATION: Biological Significance Human fascin is an actin-bundling protein, whose actin binding ability is regulated by phosphorylation. In normal tissues the detection of fascin is reported to be predominantly restricted to dendritic cells and in the thymus has been observed only in medullary dendritic cells. However, in cases of Hodgkin's disease, including nodular sclerosis, mixed cellularity lymphocyte depletion and unclassified cases, most or all Reed Sternberg cells are reported to be positive for fascin. Fascin expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.