OVERVIEW: Synonyms Monoclonal CENPH antibody, Anti-CENPH antibody, NNF1 antibody, PMF1 antibody,PMF1 antibody, NNF1, MIND kinetochore complex component, homolog antibody, kinetochore protein CENP-H antibody, centromere protein H antibody Cross Reactivity Human Applications WB Immunogen CENPH antibody was raised in Mouse using full length human recombinant protein of human CENPH produced in HEK293T cell as the immunogen. SPECIFICATIONS: Host Mouse Clone 1C2 Isotype IgG1 Method of Purification CENPH antibody was purified by affinity chromatography. Form & Buffer Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide. Concentration 0.5-1 mg/ml STORAGE & SAFETY: Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Usage Assay Information: Usage Recommendations WB: 1:2000 GENERAL INFORMATION: Biological Significance Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.