OVERVIEW: Synonyms Monoclonal HIV1-RT antibody, Anti-HIV1-RT antibody, HIV-RT-1 antibody, HIV-RT 1, HIV1-RT, HIV-1 Reverse Transcriptase antibody, HIV-RT-1, HIV1RT antibody, HIV1 RT antibody, HIV-RT 1 antibody Specificity HIV-1 RT Cross Reactivity To be determined by end-user Applications ELISA, WB Immunogen HIV1-RT antibody was raised in mouse using full length recombinant RT (HIV-1, IIIB) as the immunogen. SPECIFICATIONS: Host Mouse Clone 8C4D7 Isotype IgG1 Purity > 95% pure Form & Buffer Supplied as a liquid in PBS buffer. STORAGE & SAFETY: Storage Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -70 deg C for long term storage. Avoid repeated freeze/thaw cycles. Usage Assay Information: Usage Recommendations ELISA: 1:1,000, WB: 1:500 GENERAL INFORMATION: Biological Significance In the fields of molecular biology and biochemistry, a reverse transcriptase, also known as RNA-dependent DNA polymerase, is a DNA polymerase enzyme that transcribes single-stranded RNA into double-stranded DNA. It also helps in the formation of a double helix DNA once the RNA has been reverse transcribed into a single strand cDNA. Normal transcription involves the synthesis of RNA from DNA; hence, reverse transcription is the reverse of this.