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Exalpha/Chicken anti-native Green Fluorescent Protein/Bii-nGFPabC1-100/.1 ml
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ProductCodeProductNameChickenanti-nativeGreenFluorescentProteinSize.1mlPrice(USD)$304BackgroundGreenfluorescentprotein(GFP)isa27kDproteinwhichwasoriginallyidentifiedinthephotoorgansofAequoreavictoria(A.victoria)jellyfish.GFPisanaturallyfluorescentproteinwhichemitsgreenlightatamaximumwavelengthof509nmwhenexcitedbyblueorUVlight.InA.victoria,calciumionsbindandactivatetheproteinaequorincausingthereleaseofbluefluorescence,whichisthenabsorbedbyGFPresultinginthereleaseofgreenfluorescence.Inthelaboratory,theGFPproteinhasbeenusedextensivelyasareportermoleculetolabel,andstudy,cellularandsubcellularproteinsinlivingcellsusingawiderangeofBIOLOGicalapplications,includingoncology,cardiovasculardiseases,brainresearchandembryology,justtonameafew.Forthequantitationoftheexpressionofaspecificprotein,taggedwithGFPinthesemodelsystems,antibodiestoGFPhaveproventobeofvalueinimmunoblottingstudiesandELISAprotocols.HostChickenSpeciesReactivityn/aProductUsageTheGFPantiserumisdirectedagainstnativegreenfluorescentproteinfromA.victoriajellyfish.TheantibodyrecognisesnotonlythenativeGFP,butalsorecombinantprotein(E.Coli),nativeGFP-fusionproteinsandallvariantsofGFPtested.CrossreactivitywithE.coli.proteinsisminimal.ProductTypePrimaryAntibodiesApplicationsWesternBlotting,ELISAProductSourceTheanti-GFPantibodyisachickenpolyclonalantiserumobtainedfromwholeeggyolkofchickensimmunisedwithhighlypurifiednativeGFPderivedfromAequoreavictoriajellyfish.ProductFormulationEachvialcontains100µl1mg/mlpolyclonalantiserumin10mMTRISbufferpH8.0,containing0,15mMNaCland0.02%sodiumazide.Theantibodieswerepurifiedbyammoniumsulfateprecipitation.ProductSpecificityAequoriaVictoria(native)GFP,recombinant(E.Coli)andnativeGFP-fusionproteins,andallvariantsofGFPtested.StorageConditionsStoreat4oC,orinsmallaliquotsat-20oC.
xalpha有限公司于2000年在马萨诸塞州雪莉(美国)成立。该公司主要通过开发和生产一流的抗体来支持生命科学领域的研究人员和科学家。其领域涉及信号通路、凋亡、增殖、炎症、免疫学、传染病学和细胞间结构研究等方面。Exalpha主要产品线包括Proliferation&apoptosis, Enzymes&Proteins, Clinical IHC Markers , GPCRs等多个产品线,可提供给全球的科研实验室,制药企业,生物技术和诊断公司,医院等,目前旗下的代理公司遍布全球。
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