Product Code Product Name Mouse anti Human IgG3 (G3m(U) allotype specific), conjugated with FITC Size .2 mg Price (USD) $565 Background The reactivity of the antiserum is restricted to an allotype specific determinant on the IgG3m(u) molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG3m(u) in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG3m(u), and immunoblotting using a peroxidase labelled monoclonal antibody against FITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50; in ELISA from 1:200 upwards; in Western blotting from 1:400 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only. Host Mouse Isotype IgG1 Species Reactivity Human Product Usage Fluorescein isothiocyanate-conjugated purified monoclonal Mouse antibody to Human IgG3m(u), allotype specific. Product Type Secondary Antibodies Applications ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence Fields of Interest Immunology Product Source Highly purified monoclonal IgG3m(u) isolated from human serum. Product Formulation Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Product Specificity The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species. Storage Conditions The lyophilized product is shipped at ambient temperature and may be stored at +4