ProductDescriptionForprintingwithLifeink®200,wehighlyrecommendusingtheFRESHprintingtechniqueandprintingat2-8°C.Threedimensional(3D)gelsallowforthestudyoftheeffectsofthemechanicalpropertiesoftheextracellularmatrix(ECM),suchasdensityandrigidity,oncelldevelopment,migration,andmorphology.Unlike2Dsystems,3Denvironmentsallowcellextensionstosimultaneouslyinteractwithintegrinsonallcellsurfaces,resultingintheactivationofspecificsignalingpathways.Gelstiffnessorrigidityalsoaffectscellmigrationdifferentlyin3Dversus2Denvironments.Abioinkthatresembleslifeshouldincludethefollowingtraits:HighPrintResolution,shear-thinning,strongmechanicalstrength,superiorcytocompatibility,allowforcellularremodeling,andisbiomimetic.Lifeink®200fulfillsthoserequirements.AdvancedBiomatrixoffersLifeink®200,abioinkthatisa35mg/mltypeIcollagen.Lifeink®200ispHneutralcollagenwithphysiologicalsaltconcentration.Thecollagenstartingmaterialispurifiedusingacontrolledmanufacturingprocess.ReadthegroundbreakingpublicationusingLifeink®200forbioprintingheartcomponentshere.Lifeink®200bioinkhasthefollowingcharacteristicsasshownbelow:Parameter,Testing,andMethodLifeink®200#5278SterilizationMethodFiltration,AsepticProcessingSterility-USPmodifiedNogrowthFormOpaqueGel/PastePackageSize5mLStorageTemperature2-8°CPrintTemperature2-8°CShelfLifeMinimumof6monthsfromdateofreceiptEndotoxin-LAL<10.0EU/mLpH6.9-7.6Osmolality(mOsmoH2O/kg)290-370SourceMaterialBovineCollagenElectrophoreticPattern-CoomassieBlueCharacteristicCollagenConcentration-Biuret30-45mg/mLCollagenPurity-SilverStaining>99%ContinuousFlowExtrusionPassDirectionsforUseDownloadthefullPDFversionorcontinuereADIngbelow:Note:Employasepticpracticestomaintainthesterilityoftheproductthroughoutthepreparationandhandlingofthecollagenandothersolutions.Note:Itisrecommendedthatthecollagenandotherworkingsolutionsbechilledduringthepreparationofthecollagen.Note:Vortexingofcollagenisnotrecommendedatanystep.Note:EnsurethatNObubblesenterthesystem.Bubblesinthesystemwhilemixingwillturnyourinkintoafoam-likematerial.Note:WerecommendprintingLifeink®at2-8°Cintoagelatinslurrysupport(FRESHmethod)forbestresults.CellAdditiontoBioink:Note:KeepcollagenchilledthroughouttheseprocessesAddtheconcentratedcellsuspensionincellculturemediatoasterilesyringe.Videoforstep1Note:Werecommendafinalbioinkcellconcentrationof5X106/mLorgreater.Note:Forbestresults,add2mLofcellsuspensionper5mLofcollagenBioink.Useasimilarratioforsmallervolumes.Placesterilecouplerontheendofthesyringewiththecellsuspension.Slowlypushplungerinuntilmediaformsaslightexternalmeniscusabovetheendofthecoupleronthesyringe.Removecapfromthesyringewithcollagenandslowlypushplungerinuntilcollagenformsaslightexternalmeniscusabovetheendofthesyringe.Couplethesyringewithcellstothesyringewithcollagen.(Ensurethattherearenoairbubblesinthesystem.The“externalmeniscus”onbothsyringeshelpsensurethattherearenoairbubblesintroduced).Slowlypushplungersbackandforth~40timestoensurethoroughmixing.Endwithallofthematerialinthesyringetobeusedforprinting.Videoforsteps2-6Thecell-ladenbioinkisnowreadyforpositivedisplacementprinters.Allow5-10minutesforthesyringetoequilibratebackto2-8°Cbeforeprintingforbestresults.Note:Forpneumaticprinters,transferthecollagenintoanappropriatesyringeusingthecoupler.Thenewsyringeshouldhavethesealinserted,buttheplungerremoved.Centrifugethesyringeat2000RPMfor1minuteaftertransferringthecollagentoremoveanyairbubbles.Donotdothecentrifugestepiftherearecellsalreadymixedin.WatchthisvideoforhelpGeneralPrintingNotes:Touseasmallervolumeofcollagen,simplytransferthedesiredamountofcollagentoanothersyringe,usingtheprovidedsterilecoupler.Toremovetheairfromthenewsyringe,youcandoeitherofthefollowing:Centrifugethesyringe(capped)withthecappointinguptocausetheairtoaccumulateatthecap.Evacuatetheair.Centrifugethesyringe(capped)withthecappointingdown,andthenuseahemostattosqueezethesyringewhilepushingtheplungertoallowtheairtoescape.RemovingairwithahemostatvideoCompleteyourprintwithin1hourofcelladditiontosyringe/bioinkforbestcellviABIlityresults.WhenprintingwithFRESHgelatinslurry,allowthefinalprintedstructuretoincubateat37°Cfor30to60minutesandthenreplacethegelatinwithmedia.Avoidbubbles.ProductCellAssayFibroblastsDay2AdiposeDerivedStemCellsDay1AdiposeDerivedStemCellsDay6Fibroblastsday1Fibroblastsday5ProductBioprintsFullScaleHumanNeonatalHeartHeartValve-ClosedHeartValve-OpenMeniscusSimpleScaffoldJustahand...(Canyouseethesinglelayerthinfingernails?)PreviousNextProductReferencesReferencesforLifeink®collagenbioinks:Lee,A.etal.3Dbioprintingofcollagentorebuildcomponentsofthehumanheart.Science365,482–487(2019).Maxson,EvaL.,etal."Invivoremodelingofa3D-Bioprintedtissueengineeredheartvalvescaffold."Bioprinting(2019):e00059.Filardo,G.etal.Patient-specificmeniscusprototypebasedon3Dbioprintingofhumancell-ladenscaffold.Bone&JointResearch8,101–106(2019).Schmitt,T.AnalysisandClassificationof3-DPrintedCollagen-BioglassMatricesforCellularGrowthUtilizingArtificialNeuralNetworks.UniversityThesis(2018).Balakhovsky,Y.M.,Ostrovskiy,A.Y.&Khesuani,Y.D.EmergingBusinessModelsTowardCommercializationofBioprintingTechnology.3DPrintingandBiofabrication1–22(2017).doi:10.1007/978-3-319-40498-1_25-1Fox,S.etal.Asimplifiedfabricationtechniqueforcellularizedhigh-collagendermalequivalents.BiomedicalMaterials14,041001(2019).ProductCertificateofAnalysisNoresultfor.SafetyandDocumentationSafetyDataSheetCertificateofOriginDeclarationofMaterialSourceProductDisclaimerThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.

美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到最佳质量，并保证产品之间一致性，方便研究客户使用。

以下为Advanced BioMatrix 3D Matrices 产品竞争优势：
1. 提供高纯度和成分确定的胞外基质；
2. 超过1000余篇文献引用PureCol产品，品质非常均一；
3. 在3D培养基领域可提供最全面的产品线；
4. 唯一可提供特异性刚性有机硅基板的公司（CytoSoft）;
5. 唯一可提供可溶性丝纤蛋白的供应商（可运用于多种3D培养）；
6. 如果客户首次接触3D胶原凝胶，Advanced BioMatrix还是唯一的预制胶原蛋白(PureCol EZ Gel)供应商。

以下产品为Advanced BioMatrix全球畅销品：
1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML
2. Nutragen牛源I型胶原蛋白 6 mg/ml #5005-100ML
3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML
4. VitroCol 人源I型胶原蛋白 #5007-20ML
5. 弹性蛋白原 #5052-1MG
6. ECM Select Array kit Ultra-36 #5170-1EA
7. CytoSoft（刚性可变的基底，Advanced BioMatrix最新添加产品5190-7EA）
8. 人III型胶原蛋白 #5021-10MG
9. 人IV型胶原蛋白 #5022-5MG
10. Silk Fibroin溶液 #5154-20ML
11. Fibronectin #5080-5MG
12. Vitronectin #5051-0.1MG

Advanced BioMatrix最畅销的产品是PureCol（5005-100ML）。目前已有上千发表的论文使用这款产品。（Nutragen 5010、fibricol 5133和PureCol的物质成分一样，仅仅是浓度不同）浓度越高，胶原蛋白硬度越强。