Product DescriptionLifeink® 240 is an acidic Type I collagen bioink at a concentration of 35 mg/ml for extrusion-based 3D bioprinting. The product is a highly concentrated acidified collagen solution intended to be extrusion printed employing FRESH bioprinting (see LifeSupport® Catalog No. 5244-8GM).Lifeink® 240 produces printed structures with a high print resolution and good mechanical strength. The product is formulated in an acidic saline buffer solution. Once the collagen is printed into LifeSupport®, the pH and salts concentration of the printed structure become physiological. Cells can then be seeded onto the printed structure allowing for cell adherence and cellular remodeling of the 3D bioprinted structure.Parameter, Testing, and MethodLifeink®240 #5267Sterilization MethodFiltration, Aseptic ProcessingSterility - USP modifiedNo growthFormClear Viscous SolutionPackage Size5 mLStorage Temperature2-8°CPrint Temperature2-25°CShelf LifeMinimum of 6 months from date of receiptEndotoxin - LAL<10.0 EU/mLpH3.0-5.0Osmolality (mOsmo H2O/kg)450-700Source MaterialBovine CollagenElectrophoretic Pattern - Coomassie BlueCharacteristicCollagen Concentration - Biuret30-40 mg/mLCollagen Purity - Silver Staining>99%Continuous Flow ExtrusionPassDirections for UseDownload the full PDF version or continue reading below:Note: Employ aseptic practices to maintain the sterility of the product throughout the preparation and handling of the collagen and other solutions.Note: Ensure that NO bubbles enter the system if mixing other materials. Bubbles in the system while mixing will turn your ink into a foam-like material.Note: Cells should not be added directly to the Lifeink® 240 bioink since it is an acidic formulation.Addition of other components to Lifeink®240 Bioink:Note: Components other than cells can be added to the Lifeink® 240 bioink before bioprinting as long as these components are compatible with acidic pH conditions.To mix in additives to the bioink, add the additives to a secondary syringe as demonstrated in the video below:Video for step 1Note: The video shows cells being added to the bioink. Other materials can be added to the Lifeink® 240 bioink in lieu of cells. Cells should NOT be added directly to this bioink. For cellular bioprinting, use Lifeink® 200 #5278-5ML.Note: For best results, add no more than 5 mL of other components per 5 mL of collagen bioink. Use a similar ratio for smaller volumes.Place sterile coupler on the end of the syringe with the bioink additives.Slowly push plunger in until solution forms a slight external meniscus above the end of the coupler on the syringe.Remove cap from the syringe with collagen and slowly push plunger in until collagen forms a slight external meniscus above the end of the syringe.Couple the syringe with additives to the syringe with collagen.(Ensure that there are no air bubbles in the system. The “external meniscus” on both syringes helps ensure that there are no air bubbles introduced).Slowly push plungers back and forth ~40 times to ensure thorough mixing. End with all of the material in the syringe to be used for printing.Video for steps 2-6The bioink with other components is now ready for extrusion 3D bioprinters.Note: For pneumatic printers, transfer the collagen into an appropriate syringe using the coupler. The new syringe should have the seal inserted, but the plunger removed. Centrifuge the syringe at 2000 RPM for 1 minute after transferring the collagen to remove any air bubbles.Watch this video for helpGeneral Printing Notes:To use a smaller volume of collagen, simply transfer the desired amount of collagen to another syringe, using the provided sterile coupler. To remove the air from the new syringe, you can do either of the following:Centrifuge the syringe (capped) with the cap pointing up to cause the air to accumulate at the cap. Evacuate the air.Centrifuge the syringe (capped) with the cap pointing down, and then use a hemostat to squeeze the syringe while pushing the plunger to allow the air to escape.Removing air with a hemostat videoWhen printing with FRESH gelatin slurry, allow the final printed structure to incubate at 37°C for 30 to 60 minutes and then replace the gelatin with media.Avoid bubbles.For more directions on FRESH printing, please visit our LifeSupport® Directions for Use.Product ReferencesReferences for Lifeink®collagen bioinks:Lee, A.et al.3D bioprinting of collagen to rebuild components of the human heart.Science365,482–487 (2019).Maxson, Eva L., et al. "In vivo remodeling of a 3D-Bioprinted tissue engineered heart valve scaffold."Bioprinting(2019): e00059.Filardo, G.et al.Patient-specific meniscus prototype based on 3D bioprinting of human cell-laden scaffold.Bone & Joint Research8,101–106 (2019).Schmitt, T. Analysis and Classification of 3-D Printed Collagen-Bioglass Matrices for Cellular Growth Utilizing Artificial Neural Networks.University Thesis (2018).Balakhovsky, Y. M., Ostrovskiy, A. Y. & Khesuani, Y. D. Emerging Business Models Toward Commercialization of Bioprinting Technology.3D Printing and Biofabrication1–22 (2017). doi:10.1007/978-3-319-40498-1_25-1Fox, S.et al.A simplified fabrication technique for cellularized high-collagen dermal equivalents.Biomedical Materials14,041001 (2019).Product Certificate of AnalysisNo result for .Safety and DocumentationSafety Data SheetCertificate of OriginDeclaration of Material SourceProduct DisclaimerThis product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到最佳质量，并保证产品之间一致性，方便研究客户使用。

以下为Advanced BioMatrix 3D Matrices 产品竞争优势：
1. 提供高纯度和成分确定的胞外基质；
2. 超过1000余篇文献引用PureCol产品，品质非常均一；
3. 在3D培养基领域可提供最全面的产品线；
4. 唯一可提供特异性刚性有机硅基板的公司（CytoSoft）;
5. 唯一可提供可溶性丝纤蛋白的供应商（可运用于多种3D培养）；
6. 如果客户首次接触3D胶原凝胶，Advanced BioMatrix还是唯一的预制胶原蛋白(PureCol EZ Gel)供应商。

以下产品为Advanced BioMatrix全球畅销品：
1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML
2. Nutragen牛源I型胶原蛋白 6 mg/ml #5005-100ML
3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML
4. VitroCol 人源I型胶原蛋白 #5007-20ML
5. 弹性蛋白原 #5052-1MG
6. ECM Select Array kit Ultra-36 #5170-1EA
7. CytoSoft（刚性可变的基底，Advanced BioMatrix最新添加产品5190-7EA）
8. 人III型胶原蛋白 #5021-10MG
9. 人IV型胶原蛋白 #5022-5MG
10. Silk Fibroin溶液 #5154-20ML
11. Fibronectin #5080-5MG
12. Vitronectin #5051-0.1MG

Advanced BioMatrix最畅销的产品是PureCol（5005-100ML）。目前已有上千发表的论文使用这款产品。（Nutragen 5010、fibricol 5133和PureCol的物质成分一样，仅仅是浓度不同）浓度越高，胶原蛋白硬度越强。