ProductDescriptionAdvancedBiomatrixoffersPhotoGel®RUTKit,apurifiedmethacrylatedgelatinkitforvisIBLelight(400-450nm)photocrosslinkablehydrogels.PhotoGel®provides3Dtunablegelswiththeuniqueattributestobepreparedatvariousconcentrationsandcrosslinkedtoprovidevariousgelstiffness.ThisPhotoGel®kitconsistsofpurifiedmethacrylatedporcinegelatinandaVisibleLightPhotoinitiator(400-450nm).ThegelatinistypeA300bloom.Table1:ItemCatalogNumberPackageSizeStorageTemperatureMethacrylatedGelatin#52081gram(2x500mg)-20°CRutheniumPhotoinitiator#5246100mgRoomTemperatureSodiumPersulfatePhotoinitiator#5247500mgRoomTemperaturePhotoGel®isproducedfrommethacrylatedgelatinwherethegelatinhasbeenmodifiedbyreactingthefreeamines,primarilytheε-aminesgroupsofthelysineresiduesaswellasthea-aminesgroupsontheN-termini.>75%ofthetotallysineresiduesofthegelatinmoleculehavebeenmethacrylated.ThephotoinitiatorconsistsofRutheniumandSodiumPersulfatetobeformulatedin1XcellculturemediaorPBS,whichallowsvisiblelightphotocrosslinkingofthecollagenat400-450nm.Forasepticwork,werecommendfilteringthesolubilizedrutheniumandsodiumpersulfatethrough0.2micronbuttonfilters,asthephotoinitiatorsdonotcomesterile.Parameter,Testing,andMethodMethacrylatedGelatin#5208SterilizationMethodFiltrationSterility-USPmodifiedNogrowthFormLyophilizedPowderPackageSize1gram(2x500mg)StorageTemperature-20°CShelfLifeMinimumof6monthsfromdateofreceiptDegreeofMethacrylation>75%GelatinSourceTypeA,300Bloom,PorcineHydrogelYoung"sModulusE(Pa)CharacteristicDirectionsforUseDownloadthefullPDFversionorcontinuereADIngbelow:3DHydrogelPreparation:Note:Employasepticpracticestomaintainthesterilityoftheproductthroughoutthepreparationandhandlingofthecollagenandothersolutions.Note:Thefollowinginstructionsarefora10%gelatinmethacrylatesolution.Recommendedconcentrationsare5-20%.Warm10mLofwarm,sterile1XPBSor1Xcellculturemediato>60°C.Addthe10mL’sofwarmedsolutiontotheambervialcontaining1gramoflyophilizedgelatinmethacrylate.Mixonashakertableorrotatorplateuntilfullysolubilized.Keepwarm(>37°C)ifpossible(eg.placeyourrotatorinanincubator)tohelpwithfullsolubilization.Calculatethevolumeofphotoinitiatortoaddbymultiplyingthevolumeofsolubilizedgelatinby0.02.Iftheresultingnumberis200ul,forexample,youwilladd200ulofrutheniumand200ulofsodiumpersulfate.Solubilizetherequiredamountofruthenium(perstep4)ataconcentrationof37.4mg/mlin1XPBSorcellculturemedia.Solubilizetherequiredamountofsodiumpersulfate(perstep4)ataconcentrationof119mg/mlin1XPBSorcellculturemedia.Addtherutheniumtothegelatinsolutionandfullymixuntilsolutionishomogeneous.Addthesodiumpersulfatetothegelatin/rutheniumsolutionandmixuntilsolutionishomogeneous.Addyourcellstothegelatin/photoinitiatorsolution.Dispenseyourgelatin/photoinitiator/cellsolutionintothedesireddish(ie.6-wellplate,48-wellplate).Forphotocrosslinking,placeprintedstructuredirectlyundera400-450nmvisiblelightcrosslinkingsource.Longerexposureallowsmorecrosslinking,thougheachcelltypewithstandsdifferentdegreesoflightandfreeradicals(generatedbythephotoinitiator)thatmediatecrosslinking.Anyexcessmaterialcanberefrigeratedandstored.Thematerialwillgel.Warmbackupto>30°Cforittobecomeliquidagain.Werecommendonlyaddingphotoinitiatortotheamountofgelatintobeusedatthattime.ProductApplicationsPhotoGel®Gelatinmethacrylatecanbeusedtoformcross-linkedhydrogelsfortissueengineering[1]and3Dprinting.Thecommonformsof3DprintingusingLifeink®300includeextrusion[2][3][4],inkjet[5]andphotolithography[6]).PhotoGel®hasbeenusedforendothelialcellmorphogenesis,[7]cardiomyocytes,[8]epidermaltissue[9],injectabletissueconstructs[10],bonedifferentiation[11],andcartilageregeneration[12].Gelatinmethacrylatehasbeenexploredindrugdeliveryapplicationsintheformofmicrospheres[13]andhydrogels[14].References:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2878615/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4040163/https://www.ncbi.nlm.nih.gov/pubmed/24112804https://www.ncbi.nlm.nih.gov/pubmed/20387987https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380738/https://www.ncbi.nlm.nih.gov/pubmed/28349897/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3643201/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551408/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4608855/http://pubs.acs.org/doi/abs/10.1021/bm401533yhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252258/https://www.ncbi.nlm.nih.gov/pubmed/24590160https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4293288/http://www.ijpsonline.com/articles/preparation-and-characterization-of-gelatinpolymethacrylic-acid-interpenetrating-polymeric-network-hydrogels-as-a-phsensitive-deli.htmlProductReferencesReferencesforPhotoGel®:Rothrauff,BenjaminB.,etal."EfficacyofThermoresponsive,photocrosslinkablehydrogelsderivedfromdecellularizedtendonandcartilageextracellularmatrixforcartilagetissueengineering."Journaloftissueengineeringandregenerativemedicine12.1(2018):e159-e170.Rothrauff,BenjaminB.,etal."Anatomicalregion-dependentenhancementof3-dimensionalchondrogenicdifferentiationofhumanmesenchymalstemcellsbysolublemeniscusextracellularmatrix."Actabiomaterialia49(2017):140-151.Rothrauff,BenjaminB.,GuangYang,andRockyS.Tuan."Tissue-specificbioactivityofsolubletendon-derivedandcartilage-derivedextracellularmatricesonadultmesenchymalstemcells."Stemcellresearch&therapy8.1(2017):133.Bridge,JackChristopher,etal."Electrospungelatin-basedscaffoldsasanovel3Dplatformtostudythefunctionofcontractilesmoothmusclecellsinvitro."BiomedicalPhysics&EngineeringExpress4.4(2018):045039.Capella-Monsonís,Héctor,etal."Scaffoldsfortendontissueengineering."HandbookofTissueEngineeringScaffolds:VolumeOne.WoodheadPublishing,2019.259-298.ProductCertificateofAnalysisNoresultfor.SafetyandDocumentationSafetyDataSheetCertificateofOriginProductDisclaimerThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.

美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到最佳质量，并保证产品之间一致性，方便研究客户使用。

以下为Advanced BioMatrix 3D Matrices 产品竞争优势：
1. 提供高纯度和成分确定的胞外基质；
2. 超过1000余篇文献引用PureCol产品，品质非常均一；
3. 在3D培养基领域可提供最全面的产品线；
4. 唯一可提供特异性刚性有机硅基板的公司（CytoSoft）;
5. 唯一可提供可溶性丝纤蛋白的供应商（可运用于多种3D培养）；
6. 如果客户首次接触3D胶原凝胶，Advanced BioMatrix还是唯一的预制胶原蛋白(PureCol EZ Gel)供应商。

以下产品为Advanced BioMatrix全球畅销品：
1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML
2. Nutragen牛源I型胶原蛋白 6 mg/ml #5005-100ML
3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML
4. VitroCol 人源I型胶原蛋白 #5007-20ML
5. 弹性蛋白原 #5052-1MG
6. ECM Select Array kit Ultra-36 #5170-1EA
7. CytoSoft（刚性可变的基底，Advanced BioMatrix最新添加产品5190-7EA）
8. 人III型胶原蛋白 #5021-10MG
9. 人IV型胶原蛋白 #5022-5MG
10. Silk Fibroin溶液 #5154-20ML
11. Fibronectin #5080-5MG
12. Vitronectin #5051-0.1MG

Advanced BioMatrix最畅销的产品是PureCol（5005-100ML）。目前已有上千发表的论文使用这款产品。（Nutragen 5010、fibricol 5133和PureCol的物质成分一样，仅仅是浓度不同）浓度越高，胶原蛋白硬度越强。