Product DescriptionThis Type III Collagen product is isolated from human placenta and is purified using a multi-step process with approximately 85% Type III collagen with the remainder being comprised of Type I collagen. The product is supplied as a sterile solution with 10 mg at approximately 1 mg/ml in 0.01 N HCl, pH 2. Type III collagen product is provided in a user-friendly packaging for use and storage. This Type III collagen product is sterile filtered and is supplied as a ready to use solution.Type III Collagen provides structure and strength to connective tissue, is found many places in the body, especially skin, lung, intestinal walls and the walls of blood vessels. Collagen III is initially produced as procollagen, a protein consisting of three pro-alpha1(III) chains that form the triple-stranded, rope-like molecule. After being synthesized, the procollagen molecule is modified by the cell. Enzymes modify the amino acids lysine and proline in the protein strands by adding chemical groups that are necessary for the strands to form a stable molecule and then later to crosslink to other molecules outside the cell. The Type III procollagen molecules are released from the cell and are processed by enzymes that clip small segments off either end of the molecules to form mature collagen. The mature collagen molecules assemble into fibrils. Cross-linking between molecules produces a very stable fibril, contributing to collagen’s tissue strengthening function.Type III Collagen is typically used as a thin coating on tissue culture surfaces or as a control. Specific instructions are found in the Directions for Use. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation. Type III collagen may be used to culture a variety of cell types.Parameter, Testing, and MethodType III Collagen#5021Sterilization MethodFiltrationFormSolutionPackage Size10 mg (~10 mL)Storage Temperature2-10°CShelf LifeMinimum of 6 months from date of receiptCollagen Concentration - Biuret0.7-1.25 mg/mLpH1.8-2.5Adventitious AgentsHuman source has been tested and found negative for Hepatitis B, C and immunodeficiency virus-1.Electrophoretic Pattern - Coomassie BlueCharacteristicSterility - USP modifiedNo growthEndotoxin (LAL)<10.0 EU/mLOsmolality (mOsmo H2O/kg)<35Cell Attachment AssayPassSourceHuman PlacentaDirections for UseDownload the full PDF versionor continue reading below:Coating ProcedureNote:Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.Remove required quantity of collagen from the bottle and dispense into a dilution vessel.Dilute Type III collagen solution with a 0.01 M HCl solution water to ~50 to 100 µg/ml (~1:10).Swirl contents gently until material is completely mixed.Add appropriate amount of diluted Type III Collagen to the culture surface ensuring that the entire surface is coated.Incubate at room temperature, covered for 1-2 hours.After incubation, aspirate any remaining material.Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.Product Q & AIs the type III collagen Atelocollagen or Telocollagen?The Type III collagen undergoes an enzyme step in the purification process, resulting in Atelocollagen. Product ReferencesReferences for Type III Collagen:Drzewiecki, Kathryn E., et al. "Circular dichroism spectroscopy of collagen fibrillogenesis: a new use for an old technique."Biophysical journal111.11 (2016): 2377-2386.Cho, Soo Hyun, et al. "Meat quality traits as a function of cow maturity."Animal Science Journal88.5 (2017): 781-789.Da, Qi, et al. "Fluorescent labeling of endogenous platelets for intravital microscopy: Effects on platelet function."Microcirculation25.6 (2018): e12457.Jerrell, Rachel J., Mitchell J. Leih, and Aron Parekh. "The altered mechanical phenotype of fetal fibroblasts hinders myofibroblast differentiation."Wound Repair and Regeneration27.1 (2019): 29-38.Simmons, Chad. "The Effect of 3D Collagen Scaffolds on Regulating Cellular Responses." (2015).Product Certificate of AnalysisNo result for .Product VideosCoating Plates with CollagenVideoSee More >Coating a Glass Coverslip with CollagenVideoSee More > See More Safety and DocumentationSafety Data SheetCertificate of OriginProduct DisclaimerThis product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到最佳质量，并保证产品之间一致性，方便研究客户使用。

以下为Advanced BioMatrix 3D Matrices 产品竞争优势：
1. 提供高纯度和成分确定的胞外基质；
2. 超过1000余篇文献引用PureCol产品，品质非常均一；
3. 在3D培养基领域可提供最全面的产品线；
4. 唯一可提供特异性刚性有机硅基板的公司（CytoSoft）;
5. 唯一可提供可溶性丝纤蛋白的供应商（可运用于多种3D培养）；
6. 如果客户首次接触3D胶原凝胶，Advanced BioMatrix还是唯一的预制胶原蛋白(PureCol EZ Gel)供应商。

以下产品为Advanced BioMatrix全球畅销品：
1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML
2. Nutragen牛源I型胶原蛋白 6 mg/ml #5005-100ML
3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML
4. VitroCol 人源I型胶原蛋白 #5007-20ML
5. 弹性蛋白原 #5052-1MG
6. ECM Select Array kit Ultra-36 #5170-1EA
7. CytoSoft（刚性可变的基底，Advanced BioMatrix最新添加产品5190-7EA）
8. 人III型胶原蛋白 #5021-10MG
9. 人IV型胶原蛋白 #5022-5MG
10. Silk Fibroin溶液 #5154-20ML
11. Fibronectin #5080-5MG
12. Vitronectin #5051-0.1MG

Advanced BioMatrix最畅销的产品是PureCol（5005-100ML）。目前已有上千发表的论文使用这款产品。（Nutragen 5010、fibricol 5133和PureCol的物质成分一样，仅仅是浓度不同）浓度越高，胶原蛋白硬度越强。