Product DescriptionThis Type IV Collagen is isolated from human placenta and is purified using a multi-step process. The product is supplied as a sterile, lyophilized powder containing 5 mg of Type IV collagen per vial.Type IVCollagenis the primary collagen found in the extracellular basement membranes separating a variety of epithelial and endothelial cells. It is a major component of the dermal-epidermal junction where it is mostly found in the lamina densa. It is a heterotrimeric molecule containing two Alpha1-like and one Alpha2-like chains.Type IV Collagen is typically used as a thin coating on tissue culture surfaces. Specific instructions are found in the Directions for Use. This product is generally usedin vitroas a substrate scaffold to enhance cell attachment, adherence and proliferation. Type IV collagen may be used to culture epithelial, endothelial, muscle, nerve and many other cell types. Additionally, this product is suitable for use as a substrate for collagenase assays and positive controls.Parameter, Testing, and MethodType IV Collagen#5022Sterilization MethodIrradiationFormLyophilized PowderPackage Size5 mgStorage Temperature-20°C prior to reconstitution and 2-10°C after reconstitutionShelf LifeMinimum of 6 months from date of receiptShelf Life after Reconstitution3 monthsAdventitious AgentsHuman source has been tested and found negative for Hepatitis B, C and immunodeficiency virus-1.Electrophoretic Pattern - Coomassie BlueCharacteristicSterility - USP modifiedNo growthEndotoxin - LAL<1.0 EU/mLCell Attachment AssayPassSourceHuman PlacentaDirections for UseDownload the full PDF versionor continue reading below:CoatingProcedureReconstitute the 5 mg vial with 5 ml of cold, sterile 0.25% acetic acid and mix by pipetting up and down several times. Allow to mix at 2 to 8°C overnight. Note: The resulting solution will be slightly hazy. If there are some insoluble materials present and you wish to remove it, aseptically centrifuge the materialfor approximately 2 minutes at 3000 RPM .Dilute the product to desired concentration with sterile 0.25% acetic acid. A typical final coating concentration may be 10 to 100 µg/ cm2. Testing will likely be required to determine optimal concentrations required for different cell culture systems.Add appropriate amount of diluted product to culture surface.Incubate at room temperature, covered, for 1-2 hours. Aspirate any remaining material. Alternatively, incubate at room temperature until surface is dry.Rinse coated surface carefully with a sterile balanced salt solution. Avoid scratching surfaces.Aspirate remaining material from coated surface.8. Coated culture vessels are now ready to use. The coated culture vessels may be stored at 2 to 10°C.Product Q & AIs the type IV collagen Atelocollagen or Telocollagen?The Type IV collagen undergoes an enzyme step in the purification process, resulting in Atelocollagen. Product ReferencesReferences for Type IV Collagen:Stanton, Bruce A., et al. "Pseudomonas aeruginosa reduces VX-809 stimulated F508del-CFTR chloride secretion by airway epithelial cells."PLoS One10.5 (2015): e0127742.Kang, Yun Gyeong, et al. "A three-dimensional hierarchical scaffold fabricated by a combined rapid prototyping technique and electrospinning process to expand hematopoietic stem/progenitor cells."Biotechnology letters38.1 (2016): 175-181.Pageau, Steven C., et al. "The effect of stromal components on the modulation of the phenotype of human bronchial epithelial cells in 3D culture."Biomaterials32.29 (2011): 7169-7180.Fulcher, M. Leslie, and Scott H. Randell. "Human nasal and tracheo-bronchial respiratory epithelial cell culture."Epithelial Cell Culture Protocols. Humana Press, Totowa, NJ, 2012. 109-121.Sellgren, Katelyn L., et al. "A biomimetic multicellular model of the airways using primary human cells."Lab on a Chip14.17 (2014): 3349-3358.Ozeki, Nobuaki, et al. "Gelatin scaffold combined with bone morphogenetic protein-4 induces odontoblast-like cell differentiation involving integrin profile changes, autophagy-related gene 10, and Wnt5 sequentially in human induced pluripotent stem cells."Differentiation93 (2017): 1-14.Ozeki, Nobuaki, et al. "Differentiation of human skeletal muscle stem cells into odontoblasts is dependent on induction of α1 integrin expression."Journal of Biological Chemistry289.20 (2014): 14380-14391.Product Certificate of AnalysisNo result for .Product VideosCoating Plates with CollagenVideoSee More >Coating a Glass Coverslip with CollagenVideoSee More > See More Safety and DocumentationSafety Data SheetCertificate of OriginProduct DisclaimerThis product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

美国Advanced BioMatrix是3D组织培养、细胞检测和细胞增殖等领域实验解决方案的佼佼者。Advanced BioMatrix在分离、纯化、冻干、细胞培养和蛋白检测、多肽粘附、附着因子、基质硬度和其他3D matrix 产品开发方面有着丰富的经验。Advanced BioMatrix的研发经验和专业知识确保其产品可达到最佳质量，并保证产品之间一致性，方便研究客户使用。

以下为Advanced BioMatrix 3D Matrices 产品竞争优势：
1. 提供高纯度和成分确定的胞外基质；
2. 超过1000余篇文献引用PureCol产品，品质非常均一；
3. 在3D培养基领域可提供最全面的产品线；
4. 唯一可提供特异性刚性有机硅基板的公司（CytoSoft）;
5. 唯一可提供可溶性丝纤蛋白的供应商（可运用于多种3D培养）；
6. 如果客户首次接触3D胶原凝胶，Advanced BioMatrix还是唯一的预制胶原蛋白(PureCol EZ Gel)供应商。

以下产品为Advanced BioMatrix全球畅销品：
1. PureCol 牛源I型胶原蛋白 3 mg/ml #5005-100ML
2. Nutragen牛源I型胶原蛋白 6 mg/ml #5005-100ML
3. FibriCol 牛源I型胶原蛋白 10 mg/ml #5133-20ML
4. VitroCol 人源I型胶原蛋白 #5007-20ML
5. 弹性蛋白原 #5052-1MG
6. ECM Select Array kit Ultra-36 #5170-1EA
7. CytoSoft（刚性可变的基底，Advanced BioMatrix最新添加产品5190-7EA）
8. 人III型胶原蛋白 #5021-10MG
9. 人IV型胶原蛋白 #5022-5MG
10. Silk Fibroin溶液 #5154-20ML
11. Fibronectin #5080-5MG
12. Vitronectin #5051-0.1MG

Advanced BioMatrix最畅销的产品是PureCol（5005-100ML）。目前已有上千发表的论文使用这款产品。（Nutragen 5010、fibricol 5133和PureCol的物质成分一样，仅仅是浓度不同）浓度越高，胶原蛋白硬度越强。