TheResistantStarchTestkitfor themeasurementandanalysisofresistantstarchinplantmaterialsandstarchsamples.Measurementoftotalstarchincerealproductsbyamyloglucosidase-alpha-amylasemethod:collaborativestudy.McCleary,B.V.,Gibson,T.S.&Mugford,D.C.(1997).JournalofAOACInternational,80,571-579.LinktoArticleReadAbstractAnAmericanAssociationofCerealChemists/AOACcollaborativestudywasconductedtoevaluatetheaccuracyandreliABIlityofanenzymeassaykitprocedureformeasurementoftotalstarchinarangeofcerealgrainsandproducts.Thefloursampleisincubatedat95degreesCwithThermostablealpha-amylasetocatalyzethehydrolysisofstarchtomaltodextrins,thepHoftheslurryisadjusted,andtheslurryistreatedwithahighlypurifiedamyloglucosidasetoquantitativelyhydrolyzethedextrinstoglucose.Glucoseismeasuredwithglucoseoxidase-peroxidasereagent.Thirty-twocollaboratorsweresent16homogeneoustestsamplesas8blindduplicates.Thesesamplesincludedchickenfeedpellets,whitebread,greenpeas,high-amylosemaizestarch,whitewheatflour,wheatstarch,oatbran,andspaghetti.Allsampleswereanalyzedbythestandardprocedureasdetailedabove;4samples(high-amylosemaizestarchandwheatstarch)werealsoanalyzedbyamethodthatrequiresthesamplestobecookedfirstindimethylsulfoxide(DMSO).Relativestandarddeviationsforrepeatability(RSD(r))rangedfrom2.1to3.9%,andrelativestandarddeviationsforreproducibility(RSD(R))rangedfrom2.9to5.7%.TheRSD(R)valueforhighamylosemaizestarchanalyzedbythestandard(non-DMSO)procedurewas5.7%;thevaluewasreducedto2.9%whentheDMSOprocedurewasused,andthedeterminedstarchvaluesincreasedfrom86.9to97.2%.Twoissuesindietaryfibermeasurement.McCleary,B.V.(2001).CerealFoodsWorld,46(4),164-165.LinktoArticleReadAbstractEnzymeactivityandpurityofthesetopics,theeasiesttodealwithistheimportanceofenzymepurityandactivity.Asascientistactivelyinvolvedinpolysaccharideresearchoverthepast25years,Ihavecometoappreciatetheimportanceofenzymepurityandspecificityinpolysaccharidemodificationandmeasurement(7).Thesefactorstranslatedirectlytodietaryfiber(DF)methodology,becausethemajorcomponentsofDFarecarbohydratepolymersandoligomers.ThecommitteereportpublishedintheMarchissueofCerealFOODSWORLDrefersonlytothemethodologyformeasuringenzymepurityandactivity(8)thatleduptheAOACmethod985.29(2).Inthisworkenzymepuritywasgaugedbythelackofhydrolysis(i.e.,completerecovery)ofaparticularDFcomponent(e.g.β-glucan,larchgalactanorcitruspectin).Enzymeactivitywasmeasuredbytheabilitytocompletelyhydrolyzerepresentativestarchandprotein(namelywheatstarchandcasein).Theserequirementsandrestrictionsonenzymepurityandactivitywereadequateatthetimethemethodwasinitiallydevelopedandservedasausefulworkingguide.However,itwasrecognizedthattherewasaneedformorestringentqualitydefinitionsandassayproceduresforenzymesusedinDFmeasurements.Measurementofresistantstarch.McCleary,B.V.&Monaghan,D.A.(2002).JournalofAOACInternational,85(3),665-675.LinktoArticleReadAbstractArobustandreliablemethodwasdevelopedtomeasureresistantstarch(RS),i.e.,starchthatentersthelargeintestine.InvivoconditionswerereflectedasmuchaspossIBLewhileauser-friendlyformatwasmaintained.Parametersinvestigatedincludedα-amylaseconcentration,pHofincubation,maltoseinhibitionofα-amylase,theneedforamyloglucosidaseinclusion,theeffectofshakingandstirringondeterminedvalues,andproblemsinrecoveringandanalyzingtheRS-containingpellet.TheRSvaluesobtainedwereingoodagreementwithpublishedinvivodata.Aninterlaboratoryevaluationofthemethodhasbeencompleted(FirstActionMethod2002.02).Measurementofresistantstarchbyenzymaticdigestioninstarchandselectedplantmaterials:Collaborativestudy.McCleary,B.V.,McNally,M.&Rossiter,P.(2002).JournalofAOACInternational,85(5),1103-1111.LinktoArticleReadAbstractInterlaboratoryperformancestatisticswasdeterminedforamethoddevelopedtomeasuretheresistantstarch(RS)contentofselectedplantfoodproductsandarangeofcommercialstarchsamples.FoodmaterialsexaminedcontainedRS(cookedkidneybeans,greenbanana,andcornflakes)andcommercialstarches,mostofwhichnaturallycontain,orwereprocessedtoyield,elevatedRSlevels.ThemethodevaluatedwasoptimizedtoyieldRSvaluesinagreementwiththosereportedforinvivostudies.Thirty-sevenlaboratoriestested8pairsofblindduplicatestarchorplantmaterialsampleswithRSvaluesbetween0.6(regularmaizestarch)and64%(freshweightbasis).Formatrixesexcludingregularmaizestarch,repeatabilityrelativestandarddeviation(RSDr)valuesrangedfrom1.97to4.2%,andreproducibilityrelativestandarddeviation(RSDR)valuesrangedfrom4.58to10.9%.Therangeofapplicabilityofthetestis2-64%RS.ThemethodisnotsuitableforproductswithrandRSDRvaluesareunacceptablyhigh.Measurementofcarbohydratesingrain,feedandfood.McCleary,B.V.,Charnock,S.J.,Rossiter,P.C.,O’Shea,M.F.,Power,A.M.&Lloyd,R.M.(2006).JournaloftheScienceofFoodandAgriculture,86(11),1648-1661.LinktoArticleReadAbstractProceduresforthemeasurementofstarch,starchdamage(gelatinisedstarch),resistantstarchandtheamylose/amylopectincontentofstarch,β-glucan,fructan,glucomannanandgalactosyl-sucroseoligosaccharides(raffinose,stachyoseandverbascose)inplantmaterial,animalfeedsandfoodsaredescribed.Mostofthesemethodshavebeensuccessfullysubjectedtointerlaboratoryevaluation.AllmethodsarebasedontheuseofenzymeseitherpurifiedbyconventionalchromatographyorproducedusingmolecularBIOLOGytechniques.Suchmethodsallowspecific,accurateandreliablequantificationofaparticularcomponent.Problemsincalculatingtheactualweightofgalactosyl-sucroseoligosaccharidesintestsamplesarediscussedindetail.Anintegratedprocedureforthemeasurementoftotaldietaryfibre(includingresistantstarch),non-digestibleoligosaccharidesandavailablecarbohydrates.McCleary,B.V.(2007).AnalyticalandBioanalyticalChemistry,389(1),291-308.LinktoArticleReadAbstractAmethodisdescribedforthemeasurementofdietaryfibre,includingresistantstarch(RS),non-digestibleoligosaccharides(NDO)andavailablecarbohydrates.Basically,thesampleisincubatedwithpancreaticα-amylaseandamyloglucosidaseunderconditionsverysimilartothosedescribedinAOACOfficialMethod2002.02(RS).Reactionisterminatedandhighmolecularweightresistantpolysaccharidesareprecipitatedfromsolutionwithalcoholandrecoveredbyfiltration.RecoveryofRS(formostRSsources)isinlinewithpublisheddatafromileostomystudies.Theaqueousethanolextractisconcentrated,desaltedandanalysedforNDObyhigh-performanceliquidchromatographybyamethodsimilartothatdescribedbyOkuma(AOACMethod2001.03),exceptthatforlogisticalreasons,D-sorbitolisusedastheinternalstandardinplaceofglycerol.Availablecarbohydrates,definedasD-glucose,D-fructose,sucrose,theD-glucosecomponentoflactose,maltodextrinsandnon-resistantstarch,aremeasuredasD-glucoseplusD-fructoseinthesampleafterhydrolysisofoligosaccharideswithamixtureofsucrase/maltaseplusβ-galactosidase.Deficiencyofmaizestarch-branchingenzymeiresultsinalteredstarchfinestructure,decreaseddigestibilityandreducedcoleoptilegrowthduringgermination.Xia,H.,Yandeau-Nelson,M.,Thompson,D.B.&Guiltinan,M.J.(2011).BMCPlantBiology,11(1),95-107.LinktoArticleReadAbstractBackground:Twodistinctstarchbranchingenzyme(SBE)isoformspredatethedivergenceofmonocotsanddicotsandhavebeenconservedinplantssincethen.ThisstronglysuggeststhatbothSBEIandSBEIIprovideuniqueselectiveadvantagestoplants.However,nophenotypefortheSBEImutation,sbe1a,hadbeenpreviouslyobserved.Toexplorethisincongruitytheobjectiveofthepresentworkwastocharacterizefunctionalandmolecularphenotypesofbothsbe1aandwild-type(Wt)intheW64Amaizeinbredline.Results:Endospermstarchgranulesfromthesbe1amutantweremoreresistanttodigestionbypancreaticα-amylase,andthesbe1amutantstarchhadanalteredbranchingpatternforamylopectinandamylose.Whenkernelsweregerminated,thesbe1amutantwasassociatedwithshortercoleoptilelengthandhigherresidualstarchcontent,suggestingthatlessefficientstarchutilizationmayhaveimpairedgrowthduringgermination.Conclusions:ThepresentreportdocumentsforthefirsttimeamolecularphenotypeduetotheabsenceofSBEI,andsuggestsstronglythatitisassociatedwithalteredphysiologicalfunctionofthestarchinvivo.WebelievethattheseresultsprovideaplausiblerationalefortheconservationofSBEIinplantsinbothmonocotsanddicots,asgreaterseedlingvigorwouldprovideanimportantsurvivaladvantagewhenresourcesarelimited.ImprovementoftheAOAC2009.01totaldietaryfibremethodforbreadandotherhighstarchcontainingmatrices.Brunt,K.&Sanders,P.(2013).FoodChemistry,140(3),574-580.LinktoArticleReadAbstractThedietaryfibre(DF)contentinwheatgrainbasedfoodproductshavebeenestablishedwithboththeclassicalAOAC985.29dietaryfibreandthenewAOAC2009.01totaldietaryfibreprotocol.Thereisagoodagreementbetweenthehighmolecularweightdietaryfibre(HMWDF)contentsmeasuredwiththeAOAC2009.01methodand(DF)contentmeasuredwiththeclassicalAOAC985.29methodinwheatgrainbasedfoodproducts.WiththeAOAC2009.01methodalsoasignificantamountoflowmolarweightdietaryfibre(LMWDF),rangingfrom1%to3%w/w,wasmeasuredwhichisnotquantifiedwiththeAOAC985.29method.Withsemi-preparativeGPCtheLMWDF(DP≥3)fractionsinthewheatgrainbasedfoodproductswereisolated.ThemonosaccharidecompositionofthedissolvedLMWDFconstituentswasdetermined.Glucosewasbyfarthemostabundantmonosaccharidepresentwitharabinose,galactose,xyloseandmannoseasminorconstituents.ItappearedthattheLMWDFcontainsstillnotfullyconverteddigestiblestarch/malto-oligosaccharidefragmentswithDP≥3,whichareerroneouslyquantifiedasLMWDF.ByintroducinganextraAMGhydrolysisstepintheAOAC2009.01protocolafterevaporationoftheethanolanddissolvingtheresidueindeionisedwater,thesemalto-oligosaccharidesarefullyhydrolysedresultinginthatwayinacorrectandlowerLMWDFcontent.Sterilizationinaliquidofaspecificstarchmakesitslowlydigestibleinvitroandlowglycemicinrats.Severijnen,C.,Abrahamse,E.,VanderBeek,E.M.,Buco,A.,vandeHeijning,B.J.M.,vanLaere,K.&Bouritius,H.(2007).TheJournalofNutrition,137(10),2202-2207.LinktoArticleReadAbstractDiabeticsarerecommendedtoeatabalanceddietcontainingnormalamountsofcarbohydrates,preferablythosewithalowglycemicindex.Forsolidfoods,thiscanbeachievedbychoosingwhole-grain,fiber-richproducts.For(sterilized)liquidproducts,suchasmealreplacers,thechoicesforcarbohydratesourcesarerestrictedduetotechnologicallimitations.Starchesusuallyhaveahighglycemicindexaftersterilizationinliquids,whereaslowglycemicsugarsandsugarreplacerscanonlybeusedinlimitedamounts.Usinganinvitrodigestionassay,weidentifiedaresistantstarch(RS)source[modifiedhighamylosestarch(mHAS)]thatmightenabletheproductionofasterilizedliquidproductwithalowglycemicindex.HeatingmHASfor4–5mininliquidincreasedtheslowlydigestiblestarch(SDS)fractionattheexpenseoftheRSportion.Theeffectwastemperaturedependentandreacheditsmaximumabove120°C.Heatingat130°CsignificantlyreducedtheRSfractionfrom49to22%.Theproductremainedstableforatleastseveralmonthswhenstoredat4°C.ToinvestigatewhetherahigherSDSfractionwouldresultinalowerpostprandialglycemicresponse,thesterilizedmHASsolutionwascomparedwithrapidlydigestiblemaltodextrin.MaleWistarratsreceivedani.g.bolusof2.0gavailablecarbohydrate/kgbodyweight.Ingestionofheat-treatedmHASresultedinasignificantattenuationofthepostprandialplasmaglucoseandinsulinresponsescomparedwithmaltodextrin.mHASappearstobeastarchsourcewhich,aftersterilizationinaliquidproduct,acquiresslow-releaseproperties.Thelong-termstabilityofmHASsolutionsindicatesthatthismayprovideasuitablecarbohydratesourceforlowglycemicindexliquidproductsforinclusioninadiabetes-specificdiet.Effectsofwheatinclusionandxylanasesupplementationofthedietonproductiveperformance,nutrientretention,andendogenousintestinalenzymeactivityoflayinghens.Mirzaie,S.,Zaghari,M.,Aminzadeh,S.,Shivazad,M.&Mateos,G.G.(2012).PoultryScience,91(2),413-425.LinktoArticleReadAbstractAnexperimentwasconductedtostudytheeffectsofinclusionofawheatcultivar(highinnonstarchpolysaccharides)andxylanasesupplementationofthedietonproductiveperformance,pHofthegastrointestinaltract,nutrientretention,andintestinalenzymeactivityofHy-LineW-36layinghensfrom25to47wkofage.Theexperimentwascompletelyrandomizedwith8treatmentsarrangedfactoriallywith4levelsofwheat(0,23,46,and69%)thatcorrespondedtoadietaryarabinoxylancontentof3.0,3.3,3.6,and3.9%,withorwithoutxylanasesupplementation.Eachtreatmentwasreplicated5times.Fortheentireexperimentalperiod,eggweight(PPPPPPPPPPncontentofthediets(PPExoticcornlineswithincreasedresistantstarchandimpactonstarchthermalcharacteristics.Rohlfing,K.A.,Pollak,L.M.&White,P.J.(2010).CerealChemistry,87(3),190-193.LinktoArticleReadAbstractTenparentcornlines,includingfourmutants(dullsugary2,amylose-extendersugary2,amylose-extenderdull,andanamylose-extenderwithintrogressedGuatemalengermplasm[GUATae])andsixlineswithintrogressedexoticgermplasmbackgrounds,werecrossedwitheachothertocreate20Progenycrossestoincreaseresistantstarch(RS)asadietaryfiberincornstarchandtoprovidematerialsforthermalevaluation.Theresistantstarch2(RS2)valuesfromthe10parentlineswere18.3–52.2%andthevaluesfromthe20progenycrosseswere16.6–34.0%.The%RS2ofparentswasnotadditiveintheoffspringbutgreaterRS2inparentswascorrelatedtogreaterRS2intheprogenycrosses(r=0.63).Differentialscanningcalorimetry(DSC)measuredstarchthermalcharacteristics,revealingpositivecorrelationsofpeakgelatinizationtemperatureandchangeinenthalpywith%RS2(r=0.65andr=0.67,P≤0.05);however,%retrogradation(ameasureofRS3)andretrogradationparametersdidnotcorrelatewith%RS2.The%RS2andonsettemperatureincreasedwiththeadditionoftheaegene,likelybecauseRSdelaysgelatinization.Effectofextrusionconditionsonresistantstarchformationfrompastrywheatflour.Kim,J.H.,Tanhehco,E.J.&Ng,P.K.W.(2006).FoodChemistry,99(4),718-723.LinktoArticleReadAbstractPastrywheatflourwasextrudedundervariousconditionsoffeedmoisture(20%,40%,and60%)andscrewspeed(150,200,and250rpm),atconstantbarreltemperatureprofile(40,60,80,100,and120°C,feedporttoexitdie).Theextrudedsampleswerestoredat4°Cfor0,7,or14days,atwhichtimesresistantstarch(RS)formationwasanalyzed.Thermalandpastingpropertiesofextrudedsamplesstoredfor14dayswereanalyzedusingadifferentialscanningcalorimeterandrapidviscoanalyzer(RVA),respectively.TheRScontentincreasedafterextrusioncomparedtonon-extrudedpastrywheatflour.Highsignificantpositivecorrelationsoffeedmoisture(PPResistantstarchandstarchpastingpropertiesofastarchsynthaseIIa-deficientwheatwithapparenthighamylose.Yamamori,M.,Kato,M.,Yui,M.&Kawasaki,M.(2006).AustralianJournalofAgriculturalResearch,57(5),531-535.LinktoArticleReadAbstractThebreadwheat(TriticumaestivumL.)analysedinthisstudyhasbeenproducedbygeneticallyeliminatingthestarchsynthaseIIaandshowsapparenthighamylose(HA)intheflourstarch.SomestarchpropertiesoftheHAwheatwereanalysed.TheHAwheatcontained2.8–3.6%resistantstarch(RS),muchmorethanthenormal(control)wheat,whichcontainedalmostnoRS.AutoclavingtheHAandnormalwheatstarchesincreasedRS.Theformercontained10.5%RSandthelatter5.9or6.8%RS.SwellingoftheHAwheatstarchanditspastingpropertiesusingRapidViscoAnalyzer(RVA)wereinvestigated.Swellingpower(g/g)oftheHAwheatin0.1%AgNO3andswellingvolume(mL/g)inureasolutionweresignificantlylessthanthoseofthenormalwheat.TheRVAprofileoftheHAwholemealandstarchalsodifferedfromthenormal.Thepeakviscosity,minimumviscosity,andfinalviscosityofHAwerelow,andbreakdown(peakminusminimumviscosity)wasverysmall.ThesefindingsshowthatamountofresistantstarchandpastingpropertiesareuniqueintheHAwheatstarch,probablycausedbylackofstarchsynthaseIIa.Effectsofprocessvariablesandadditionofpolydextroseandwheyproteinisolateonthepropertiesofbarleyextrudates.Kirjoranta,S.,Solala,K.,Suuronen,J.P.,Penttilä,P.,Peura,M.,Serimaa,R.,Tenkanen,M.&Jouppila,K.(2012).InternationalJournalofFoodScience&Technology,47(6),1165-1175.LinktoArticleReadAbstractExtrusioncookingiscommonlyusedintheproductionofsnacks.Inthepresentstudy,extrudateswerepreparedusingbarleyflouraloneandwiththeadditionofeitherpolydextrose(PD)orwheyproteinisolate(WPI)andbothPDandWPI.Independentprocessvariableswerewatercontentofthemass(17%,20%and23%),screwspeed(200,350and500rpm)andtemperatureofsection6anddie(110,130and150°C).Expansion,hardness,watercontent,porosityandchemicalcompositionoftheextrudateswereanalysed.HighlyporousandexpandedsnackproductswithhighdietaryfibreandproteincontentswereobtainedfrombarleyflourandWPIwhenwatercontentofmasswas17%,screwspeed500rpmandtemperatureofsection6anddie130°C.BarleyflouraloneorwithPDresultedinhardandnon-expandedextrudates.Expansionofextrudateswasstatisticallysignificantlyincreasedwithdecreasingwatercontentofthemassandincreasingscrewspeedinalltrials.EliminationofresistantstarchtypeIIwithintheframeworkoftotalstarchanddietaryfibreanalysisbymicrowaveirrADIation.Themeier,H.,Hollmann,J.,Neese,U.&Lindhauer,M.G.(2010).QualityAssuranceandSafetyofCrops&Foods,2(1),46-51.LinktoArticleReadAbstractIntroductionThepresenceofresistantstarchinsamplescontainingnon-starchpolysaccharideshasalwaysbeenachallengetoenzymatictotalstarchandtotalfibreanalysis.ObjectiveandmethodsBasedonmicrowave-inducedpressuredisintegrationtechniquetheAssociationofOfficialAnalyticalChemistsmethodsforthedeterminationoftotalstarch(AOAC996.11)andtotaldietaryfibre(AOAC991.43)havebeenmodifiedtocompletelyeliminateundesirableresistantstarchfractionswithrespecttodigestionproceduresusingthermostableα-amylaseandamyloglucosidase.ResultsMicrowavetreatmentofhigh-amylosestarchsamplesresultedinexcellenttotalstarchrecoveryintheAssociationofOfficialAnalyticalChemistsstandardmethodno.996.11.AfterintegrationofmicrowavedisintegrationtechniqueintothetotaldietaryfibremethodAOAC911.43irradiationexperimentswithdifferentmodelmixturesconsistingofnon-starchpolysaccharidescomponentsandhigh-amylosestarchfractionsresultedinthecompleteeliminationofundesirableresistantstarchfractions.ConclusionThereforethemicrowavetechniquecanbeaveryefficientmeansfortheeliminationofresistantstarchandprovidesmorerealisticvaluesinanalyticaltotaldietaryfibreprocedureswithrespecttosamplescontainingcriticalenzymeresistantstarches.ResistantstarchinMicronesianbananacultivarsoffershealthbenefits.Thakorlal,J.,Perera,C.O.,Smith,B.,Englberger,L.&Lorens,A.(2010).PacificHealthDialog,16(1),49-59.LinktoArticleReadAbstractResistantStarch(RS)isatypeofstarchthatisresistanttostarchhydrolyzingenzymesinthestomachandthusbehavesmorelikedietaryfibre.RShasbeenshowntohavebeneficialeffectsindiseasepreventionincludingmodulationofglycaemicindexdiabetes,cholesterolloweringcapabilityandweightmanagement,whicharecriticallyimportantformanypeopleintheFederatedStatesofMicronesia.GreenbananasareknowntocontainsubstantialconcentrationsofRSandareacommonpartoftheMicronesiandiet.ThereforetheaimofthisstudywastodeterminetheRScontentinbananacultivarsfromPohnpei,Micronesia:Daiwang,Inahsio,Karat,UtinKerenisandUtinRuk,forwhichnosuchinformationwasavailable.UtinKerenis,InahsioandUtinRukwerefoundtocontainthehighestamountsofRS.ThefateofRSafterincorporationintoafoodproduct(i.e..pancakes)wasalsostudiedandasignificantreductionintheRScontentwasfoundforeachcultivaraftercooking.Microscopyofthebananasamplesindicatedthattheoverallmorphologyofthecultivarswassimilar.Inconclusion,greenbanana,includingthesevarieties,shouldbepromotedinMicronesiaandotherplacesfortheirrichRScontentandrelatedhealthbenefitsincludingdiabetescontrol.FurtherresearchisneededtomoreclearlydeterminetheeffectsofcookingandfoodprocessingonRS.Enzymesusceptibilityofhigh-amylosestarchprecipitatedfromsodiumhydroxidedispersions.Evans,A.&Thompson,D.B.(2008).CerealChemistry,85(4),480-487.LinktoArticleReadAbstractTypeIIIresistantstarch(RS)isunderstoodtobeduetotheorderedstructureformationintheprocessofretrogradation.Mosttreatmentsofgranularhigh-amylosemaizestarch(HAMS)donotcompletelyeliminatetheoriginalorderedstructure.WehypothesizedthatresidualorderedstructurewouldconstrainsubsequentphysicalreassociationofchainsandtheformationofRS.Theobjectivewastogeneratedifferencesinenzymesusceptibilityusingtwomeansofprecipitationoffullydispersedstarchandtorelatedifferencesinenzymesusceptibilitytothestructureoftheprecipitates.Dispersionsinsodiumhydroxidewereprecipitatedeitherwithethanolorammoniumsulfate.RSandthetimecourseofdigestionweredetermined.Crystallinityandhelicitywereestimatedusingwide-angleX-raydiffractionandsolid-state13CCP/MASNMR,respectively.PrecipitationofwholestarchwithethanolledtolowerRSvalues(≈24%)thanprecipitationwithammoniumsulfate(≈39%)andalsotohigherreactionrateconstantsforanearlycomponentofdigestion.EthanolprecipitationofabranchedstarchfractiongaveessentiallynoRS,whereasammoniumsulfateprecipitationofthesamebranchedmaterialhad>20%RS.Ethanolprecipitatescontainedsinglehelices,inmostbutnotallcases,contributingtoV-typecrystallinity.AmmoniumsulfateprecipitateshaddoublehelicescontributingtoB-typecrystallinity.Invitrofermentationofspentturmericpowderwithamixedcultureofpigfaecalbacteria.Han,K.H.,Azuma,S.&Fukushima,M.(2014).Food&Function,10,2446-2452.LinktoArticleReadAbstractThefermentationpotentialofspentturmericwasstudiedininvitroswinefaecalbatchcultures.Thespentturmericresidue(theenzyme-resistantfractionfromspentturmeric,EST)wasobtainedthroughtheuseofthedigestiveenzymesamyloglucosidaseandpancreatinandcomparedtocelluloseandhigh-amylosestarch(HAS)ascarbonsources.ESTshowedsignificantincreasesintotalanaerobes,bifidobacteria,lactobacilliandlacticacidbacteriapopulationscomparedtocelluloseat12,24and48h,andthetotalanaerobiclevelintheHASgroupwassignificantlyhigherthaninthecellulosegroupat24and48h.However,asignificantdecreaseinthecoliformpopulationwasonlyfoundintheHASgroupcomparedtothecellulosegroupat48h.Thetotalshort-chainfattyacid(SCFA)concentrationsintheESTandHASgroupsweresignificantlyhigherthanthatinthecellulosegroupat12hand48h.However,therewasnosignificantdifferenceinthetotalSCFAconcentrationbetweentheESTandHASgroupsat12hand48h.AmmoniaandpHlevelsintheESTandHASgroupsweresignificantlylowerthanthoseinthecellulosegroupat24and48h,buttherewasnosignificantdifferencebetweentheESTandHASgroups.Theseresultsindicatethatthefermentationpotentialoftheenzyme-resistantfractionfromspentturmericiscomparabletothatofcommerciallyestablishedresistantstarch.Impactofancientcereals,pseudocerealsandlegumesonstarchhydrolysisandantiradicalactivityoftechnologicallyviableblendedbreads.Collar,C.,Jiménez,T.,Conte,P.&Fadda,C.(2014).CarbohydratePolymers,113,149-158.LinktoArticleReadAbstractWheatflourreplacementfrom22.5%upto45%byincorporationofternaryblendsofteff(T),greenpea(GP)andbuckwheat(BW)floursprovidedtechnologicallyviableandacceptablesensoryratedmultigrainbreadswithsuperiornutritionalvaluecomparedtothe100%wheatflour(WT)counterparts.Blendedbreadsexhibitedsuperiornutritionalcomposition,largeramountsofbioaccessiblepolyphenols,higheranti-radicalactivity,andlowerandslowerstarchdigestibility.Simultaneouslowerrapidlydigestiblestarch(57.1%)andhigherslowlydigestiblestarch(12.9%)andresistantstarch(2.8%)contents(gper100gfreshbread),consideredsuitablenutritionaltrendsfordietarystarchfractions,weremetbytheblendformulated7.5%T,15%GP,15%BK.Theassociatedmixturethatreplaced37.5%WT,showedaratherlowerextentandslowerrateofstarchhydrolysiswithmedium-lowvaluesforC∞,andH90,andlowestk,andintermediateexpectedGlycaemicIndex(86).Allmultigrainbreadscanbelabelledassourceofdietaryfibre(≥3gdietaryfibre/100gbread).CharacterizationandPrebioticEffectoftheResistantStarchfromPurpleSweetPotato.Zheng,Y.,Wang,Q.,Li,B.,Lin,L.,Tundis,R.,Loizzo,M.R.,Zheng,B.&Xiao,J.(2016).Molecules,21(7),932.LinktoArticleReadAbstractPurplesweetpotatostarchisapotentialresourceforresistantstarchproduction.Theeffectsofheat-moisturetreatment(HMT)andenzymedebranchingcombinedheat-moisturetreatment(EHMT)onthemorphological,crystallinityandthermalpropertiesofPSPstarcheswereinvestigated.Theresultsindicatedthat,afterHMTorEHMTtreatments,nativestarchgranuleswithsmoothsurfacewasdestroyedtoformamorecompact,irregularandsheet-likestructure.ThecrystallinepatternwastransformedfromC-typetoB-typewithdecreasingrelativecrystallinity.Duetostrongercrystallitesformedinmodifiedstarches,theswellingpowerandsolubilityofHMTandEHMTstarchweredecreased,whilethetransitiontemperaturesandgelatinizationenthalpyweresignificantlyincreased.Inaddition,HMTandEHMTexhibitedgreatereffectsontheproliferationofbifidobacteriacomparedwitheitherglucoseorhighamylosemaizestarch.Metabolomicandtranscriptomicresponsesinducedintheliversofpigsbythelong-termintakeofresistantstarch.Metabolomicandtranscriptomicresponsesinducedintheliversofpigsbythelong-termintakeofresistantstarch.Sun,Y.,Yu,K.,Zhou,L.,Fang,L.,Su,Y.&Zhu,W.(2016).Journalofanimalscience,94(3),1083-1094.LinktoArticleReadAbstractThepresentstudyinvestigatedmetabolomicandtranscriptomicresponsesintheliversofpigstoevaluatetheeffectsofresistantstarchonthebody’smetabolismattheextraintestinallevel.Thirty-sixDuroc×Landrace×LargeWhitegrowingbarrows(70dofage)wererandomlyallocatedtoeitherthecornstarch(CS)grouportherawpotatostarch(RPS)groupwitharandomizedcompleteblockdesign;eachgroupconsistedof6replicates(pens),with3pigsperpen.PigsintheCSgroupwereofferedacorn–soybean–baseddiet,whereaspigsintheRPSgroupwereputonadietinwhich230(growing)or280g/kg(finishing)purifiedCSwasreplacedwithpurifiedRPSduringa100-dtrial.Theliversofpigswerecollectedformetabolomeandgeneexpressionanalysis.Gaschromatography–massspectrometryanalysisshowedthatcomparedwiththeCSdiet,theRPSdietdecreased(PPPPtransmembraneglycoproteinclusterofdifferentiation36(CD36),carnitinepalmitoyltransferase1B(CPT1B),andmedium-chainacyl-CoAdehydrogenase(ACADM)weredownregulated(P1-acylglycerol-3-phosphateO-acyltransferase4(AGPAT4),glycerol-3-phosphateacyltransferase(GPAT),andfattyacidbindingprotein1(FABP1)wereupregulated(PValidationofMethodsAACCMethod32-40.01AOACMethod2002.02CODEXMethodTypeIIColourimetricmethodforthedeterminationofResistantStarchincerealproductsandfeedsPrinciple:               (α-amylase+amyloglucosidase)(1)Non-resistantstarch+H2O→D-glucose+maltose(trace)(2)Aqueousethanolwash+centrifugationtoremoveD-glucose+   maltose(3)DissolutionofresistantstarchpelletinKOHandneutralisation                     (α-amylase+amyloglucosidase)(4)Dissolvedresistantstarch+H2O→D-glucose                (glucoseoxidase)(5)D-Glucose+H2O+O2→D-gluconate+H2O2                                          (peroxidase)(6)2H2O2+p-hydroxybenzoicacid+4-aminoantipyrine→                                     quinoneimine+4H2OKitsize:                        100assaysMethod:                         Spectrophotometricat510nmReactiontime:                 ~120min(plusovernightincubation)Detectionlimit:                2-100%ofsampleweightApplicationexamples:Plantmaterials,starchsamplesandothermaterialsMethodrecognition:   AOAC(Method2002.02),AACC(Method32-40.01)andCODEX(TypeIIMethod)AdvantagesVerycosteffective Allreagentsstablefor>2yearsafterpreparation Onlyenzymatickitavailable Measuresenzymeresistantstarch Simpleformat Mega-Calc™softwaretoolisavailablefromourwebsiteforhassle-freerawdataprocessing Standardincluded

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