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Prosci/14 3 3 zeta (phospho Ser58) Antibody/79-188/0.1 mg
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SpecificationsSPECIESREACTIVITY:Human,Mouse,RatTESTEDAPPLICATIONS:IF,IHC,WBAPPLICATIONS:WesternBlot:1:500~1:1000,Immunohistochemistry:1:50~1:100,Immunofluorescence:1:100~1:200PREDICTEDMOLECULARWEIGHT:28kDaSPECIFICITY:Thisantibodydetectsendogenouslevelof14-3-3zetaonlywhenphosphorylatedatserine58.IMMUNOGEN:14-3-3zeta(Phospho-Ser58)antibodywasraisedagainstapeptidesequencearoundphosphorylationsiteofserine58(R-S-S(p)-W-R)derivedfromHuman14-3-3zeta.HOSTSPECIES:RabbitPropertiesPURIFICATION:Antibodieswerepurifiedbyaffinity-chromatographyusingepitope-specificphosphopeptide.Non-phosphospecificantibodieswereremovedbychromatogramphyusingnon-phosphopeptide.PHYSICALSTATE:LiquidBUFFER:Antibodysuppliedinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.CONCENTRATION:1mg/mLSTORAGECONDITIONS:Storeantibodyat-20˚Cforuptooneyear.CLONALITY:PolyclonalCONJUGATE:UnconjugatedAdditionalInfoALTERNATENAMES:HEL4,YWHAD,KCIP-1,HEL-S-3,14-3-3-zeta,JNK2,PRKM9,SAPK1A,1433Z,143Z,FAS,FactoractivatingexoenzymeSACCESSIONNO.:NP_001129171.1PROTEINGINO.:208973238OFFICIALSYMBOL:YWHAZGENEID:7534BackgroundBACKGROUND:Thisgeneproductbelongstothe14-3-3familyofproteinswhichmediatesignaltransductionbybindingtophosphoserine-containingproteins.Thishighlyconservedproteinfamilyisfoundinbothplantsandmammals,andthisproteinis99%identicaltothemouse,ratandsheeporthologs.TheencodedproteininteractswithIRS1protein,suggestingaroleinregulatinginsulinsensitivity.Severaltranscriptvariantsthatdifferinthe5"UTRbutthatencodethesameproteinhavebeenidentifiedforthisgene.REFERENCES:1)GuYM,etal.(2006)FEBSLett;580(1):305-310PowellDW,etal.(2003)MolCellBiol;23(15):5376-5387MackintoshC.(2004)BiochemJ;381(Pt2):329-342.

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