产品说明
SpecificationsSPECIES:MurineSOURCESPECIES:E.coliSEQUENCE:DGNTTRTPETNGSLCGAPGENCTGTTPRQKVKTHFSRCPKQYKHYCIHGRCRFVVDEQTPSCICEKGYFGARCERVDLFYTESTEDAPPLICATIONS:BIOLOGICALACTIVITY:BiologicalactivitywasdeterminedbyitsABIlitytostimulatetheproliferationofmouseBalb/3T3cells.TheexpectedED50is≤0.01ng/mL,correspondingtoaspecificactivityof≥1x108units/mg.Endotoxinlevelislessthan0.1ngperμg(1EU/μg).PropertiesPURITY:Greaterthan98%bySDS-PAGEgelandHPLCanalyses.PHYSICALSTATE:LyophilizedSTORAGECONDITIONS:ThelyophilizedBetacellulinrecombinantproteinisstableforatleast2yearsfromdateofreceiptat-20˚C.ReconstitutedBetacellulinstableforatleast3monthswhenstoredinworkingaliquotswithacarrierproteinat-20˚C.Aswithanyprotein,exposingBetacellulinrecombinantproteintorepeatedfreeze/thawcyclesisnotrecommended.Whenworkingwithproteinscareshouldbetakentokeeprecombinantproteinatacoolandstabletemperature.AdditionalInfoALTERNATENAMES:Bcn,Bcn,Probetacellulin,BTCACCESSIONNO.:NP_031594.1PROTEINGINO.:6680812BackgroundBetacellulinisanEGF-relatedpolypeptidegrowthfactorthatsignalsthroughtheEGFreceptor.Itisproducedinseveraltissues,includingthepancreas,smallintestine,andincertaintumorcells.Betacellulinisapotentmitogenforretinalpigmentepithelialcellsandvascularsmoothmusclecells.Betacellulinisinitiallysynthesizedasaglycosylated32.0kDatransmembraneprecursorprotein,whichisprocessedbyproteolyticcleavagetoproducethematuresequence.RecombinantmurineBetacellulinisa9.0kDamonomericprotein,containing80aminoresidues,whichcomprisesthematureEGFhomologousportionoftheBetacellulinprotein.
ProSci
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