SpecificationsTESTEDAPPLICATIONS:ELISASETCONTENTS:Thissetincludeacapturemonoclonalantibody[7F4],abiotin-labeleddetectionmonoclonalantibody[8G9],andarecombinantHIV-1p24proteinstandard.APPLICATIONS:ThissetcanbeusedtomonitortherateofHIV-1productioninvitroorforthedetectionofHIV-1.Itcandetectp24fromdifferentHIV-1subtypeswithcomparablesensitivity.ThedetectionsethasbeenoptimizedandvalidatedforHIVresearch.CaptureAntibody:HIV-1p24Antibody[7F4],Cat.No.PM-6585Thisantibodywasraisedagainstarecombinantfull-lengthHIV-1p24protein.Thesuggestedcoatingconcentrationinap24sandwichELISAis5-10µg/ml.DetectionAntibody:HIV-1p24Antibody[8G9],Cat.No.PM-6335-biotinThisantibodywasraisedagainstarecombinantfull-lengthHIV-1p24proteinandconjugatedwithbiotin.Thesuggestedconcentrationinasandwichp24ELISAis0.125-0.25µg/ml.ProteinStandard:HIV-1p24RecombinantProtein,Cat.No.95-153Thisproteinistherecombinantfull-lengthHIV-1p24fromHxB2strain(aa133-364)expressedandpurifiedfromSaccharomycescerevisiae.Therecombinantproteinisusedasastandardforcalibrationofthep24ELISA.USERNOTE:Setisavailablein3differentsizeswithenoughreagentsfor1,5,or1096-wellplates.SetContentsPropertiesPHYSICALSTATE:LiquidSTORAGECONDITIONS:StoreHIV-1p24MonoclonalAntibodiesat-20˚C.StoreHIV-1p24recombinantproteinat-70˚C.Avoidrepeatedfreeze/thawcycles.AdditionalInfoBackgroundThehumanimmunodeficiencyvirustype1(HIV-1)particleconsistsofanenvelope,acoreandtheregionbetweenthetwotermedmatrix(1).TheHIV-1Gagproteinisalatestructuralproteinthatcontainsfourproteins:matrix(p17),capsid(p24),nucleocapsid(p7)andthep6protein(2).Thep24constitutesthemajorcorecomponentofthevirusandshowshighdegreeofsequenceconservationamongHIVisolates.TheGagp24hasbeenusedasanintegralpartofmulticomponentHIV-1vaccines(3).References:1)PMID:9684349GotoT,NakaiM,andIkutaK.Thelife-cycleofhumanimmunodeficiencyvirustype1.Micron1998;29:123-38.2)PMID:9813197FreedEO.HIV-1gagproteins:diversefunctionsintheviruslifecycle.Virology1998;251:1-15.3)PMID:21467219FlynnBJ,KastenmullerK,Wille-ReeceU,etal.ImmunizationwithHIVGagtargetedtodendriticcellsfollowedbyrecombinantNewYorkvacciniavirusinducesrobustT-cellimmunityinnonhumanprimates.PNAS2011;108(17):7131–6.