SpecificationsSPECIESREACTIVITY:Bovine,Dog,Human,Mouse,Rat,Xenopus,ZebrafishHOMOLOGY:1345591TESTEDAPPLICATIONS:WBAPPLICATIONS:TheantibodyhasbeendirectlytestedforreactivityinWesternblotswithrattissue.Itisanticipatedthattheantibodywillreactwithbovine,canine,chicken,human,mouse,non-humanprimates,Xenopusandzebrafishbasedonthefactthatthesespecieshave100%homologywiththeaminoacidsequenceusedasantigen.USERNOTE:Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher.PREDICTEDMOLECULARWEIGHT:29IMMUNOGEN:PeptidecorrespondingtoaminoacidresiduesfromtheC-terminalregionof14-3-3protein,conjugatedtokeyholelimpethemocyanin(KLH).HOSTSPECIES:RabbitPropertiesPURIFICATION:AffinityPurifiedPHYSICALSTATE:LiquidBUFFER:100uLin10mMHEPES(pH7.5),150mMNaCl,100ugpermLBSAand50%glycerol.STORAGECONDITIONS:14-3-3antibodycanbestoredat-20˚Candisstableat-20˚Cforatleast1year.CLONALITY:PolyclonalCONJUGATE:UnconjugatedAdditionalInfoALTERNATENAMES:PrepronervegrowthfactorRNH-1,KCIP-1ACCESSIONNO.:P35213OFFICIALSYMBOL:YwhabGENEID:56011BackgroundBACKGROUND:14-3-3proteinsareafamilyofhighlyconservedproteinsthatappeartohavemultiplerolesincellsignaling(BridgesandMoorhead,2005).Theproteinsareabundantlyexpressedinthebrainandhavebeendetectedinthecerebrospinalfluidofpatientswithdifferentneurologicaldisorders(Bergetal.,2003).14-3-3proteinsbindproteinligandsthataretypicallyphosphorylatedonserineorthreonineresiduesandregulatethefunctionsofthesebindingpartnersbyanumberofdifferentmechanisms(Silhanetal.,2004;DoughertyandMorrison,2004).The14-3-3proteinsaffectadiversearrayofcellularprocessesincludingthecellcycleandtranscription,signaltransductionandintracellulartrafficking.REFERENCES:1)BergD,HolzmannC,RiessO(2003)14-3-3Proteinsinthenervoussystem.NatRevNeurosci4:752-762.2)BridgesD,MoorheadGB(2005)14-3-3Proteins:anumberoffunctionsforanumberedprotein.SciSTKE2005:re10.3)DoughertyMK,MorrisonDK(2004)Unlockingthecodeof14-3-3.JCellSci117:1875-1884.