SpecificationsSPECIES:MouseSOURCESPECIES:HEK293cellsSEQUENCE:Val24-Leu211FUSIONTAG:FcTagTESTEDAPPLICATIONS:ELISAAPPLICATIONS:ThisrecombinantproteincanbeusedforE,WB.Forresearchuseonly.BIOLOGICALACTIVITY:MeasuredbyitsbindingABIlityinafunctionalELISA.ImmobilizedMouseB7-1,FcTagat2ug/mL(100ul/well),canbindMouseCTLA-4,HisTagwithalinearrangeof0.02-0.8ng/mL(QCtested).PropertiesPURITY:>92%asdeterminedbySDS-PAGE.Endotoxinlevelislessthan1.0EUperugbytheLALmethod.PREDICTEDMOLECULARWEIGHT:46.7kDaPHYSICALSTATE:LyophilizedBUFFER:50mMTris,100mMGlycine,pH7.5STORAGECONDITIONS:LyophilizedProteinshouldbestoredat-20˚Corlowerforlongtermstorage.Uponreconstitution,workingaliquotsshouldbestoredat-20˚Cor-70˚C.Avoidrepeatedfreeze-thawcycles.AdditionalInfoALTERNATENAMES:4-1BBACCESSIONNO.:NP_001070976Background4-1BBisalsoknownasCD137,tumornecrosisfactorreceptorsuperfamilymember9(TNFRSF9),inducedbylymphocyteactivation(ILA),isaco-stimulatorymoleculeofthetumornecrosisfactor(TNF)receptorsuperfamily.CD137canbeexpressedbyactivatedTcells,buttoalargerextentonCD8thanonCD4Tcells.Inaddition,CD137expressionisfoundondendriticcells,folliculardendriticcells,naturalkillercells,granulocytesandcellsofbloodvesselwallsatsitesofinflammation.ThebestcharacterizedactivityofCD137isitscostimulatoryactivityforactivatedTcells.CrosslinkingofCD137enhancesTcellproliferation,IL-2secretionsurvivalandcytolyticactivity.Further,itcanenhanceimmuneactivitytoeliminatetumorsinmice.CD137canenhanceactivation-inducedTcellapoptosiswhentriggeredbyengagementoftheTCR/CD3complex.Inaddition,4-1BB/4-1BBLco-stimulatorypathwayhasbeenshowntoaugmentsecondaryCTLresponsestoseveralviruses,andmeanwhileaugmentanti-tumorimmunity.4-1BBthusisapromisingcandidateforimmunotherapyofhumancancer.CD137hasbeenshowntointeractwithTRAF2.