SpecificationsSPECIESREACTIVITY:Human,MouseHOMOLOGY:Predictedspeciesreactivitybasedonimmunogensequence:Rat:(100%),Bovine:(92%)TESTEDAPPLICATIONS:ELISA,ICC,IF,WBAPPLICATIONS:4E-BP1antibodycanbeusedforthedetectionof4E-BP1byWesternblotat2.5-10μg/mL.Antibodycanalsobeusedforimmunocytochemistrystartingat2μg/mL.Forimmunofluorescencestartat20μg/mL.USERNOTE:Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher.POSITIVECONTROL:1)Cat.No.1212-3T3CellLysate 2)Cat.No.17-201-3T3/BALBCellSlideIMMUNOGEN:4E-BP1antibodywasraisedagainsta14aminoacidsyntheticpeptidefromnearthecarboxyterminusofhuman4E-BP1.Theimmunogenislocatedwithinthelast50aminoacidsof4E-BP1.HOSTSPECIES:RabbitPropertiesPURIFICATION:4E-BP1Antibodyisaffinitychromatographypurifiedviapeptidecolumn.PHYSICALSTATE:LiquidBUFFER:4E-BP1AntibodyissuppliedinPBScontaining0.02%sodiumazide.CONCENTRATION:1mg/mLSTORAGECONDITIONS:4E-BP1antibodycanbestoredat4˚Cforthreemonthsand-20˚C,stableforuptooneyear.Aswithallantibodiescareshouldbetakentoavoidrepeatedfreezethawcycles.Antibodiesshouldnotbeexposedtoprolongedhightemperatures.CLONALITY:PolyclonalISOTYPE:IgGCONJUGATE:UnconjugatedAdditionalInfoALTERNATENAMES:4E-BP1Antibody:BP-1,4EBP1,4E-BP1,PHAS-I,Eukaryotictranslationinitiationfactor4E-bindingprotein1,Phosphorylatedheat-andacid-stableproteinregulatedbyinsulin1ACCESSIONNO.:NP_004086PROTEINGINO.:4758258OFFICIALSYMBOL:EIF4EBP1GENEID:1978BackgroundBACKGROUND:4E-BP1Antibody:ThetranslationofmRNAineukaryoticcellsisregulatedbythepresenceofaminoacidsthroughmultiplemechanisms.Onesuchmechanisminvolvestheevolutionarilyconservedserine/threoninekinaseTOR(Targetofrapamycin,alsoknownasmTOR),whichregulatescellgrowthandcellcyclethroughitsABIlitytointegratesignalsfromnutrientlevelsandgrowthfactors.OnedownstreamtargetofTORistheeukaryoticinitiationfactor4Ebindingprotein1(4E-BP1)whosephosphorylationpreventsitsassociationwitheIF4E,preferentiallystimulatingtranslationofmRNAscontaininglong,highlystructured5"-UTRs.RapamycininhibitsTORresultinginreducedcellgrowthandreducedratesofcellcycleandcellproliferationl,atleastinpartbyinhibitingtheactivityofTORtowards4E-BP1.REFERENCES:1)ShahOJ,AnthonyJC,KimballSR,etal.4E-BP1andS6K1:translationalintegrationsitesfornutritionalandhormonalinformationinmuscle.Am.J.Physiol.Endocrinol.Metab.2000;279:E715-29.2)ShamjiAF,NgheimP,andSchreiberSL.Integrationofgrowthfactorandnutrientsignaling:implicationsforcancerBIOLOGy.Mol.Cell2003;12:271-80.