SpecificationsSPECIESREACTIVITY:Human,RatTESTEDAPPLICATIONS:WBAPPLICATIONS:TheantibodyhasbeendirectlytestedforreactivityinWesternblotswithratandhumantissue.Itisanticipatedthattheantibodywillreactwithnonhumanprimatesbasedonthefactthatthesespecieshave100%homologywiththeaminoacidsequenceusedasantigen.USERNOTE:Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher.PREDICTEDMOLECULARWEIGHT:80IMMUNOGEN:Phosphopeptidecorrespondingtoaminoacidresiduessurroundingthephospho-Ser523ofhuman5-lipoxygenase(5-LO).HOSTSPECIES:RabbitPropertiesPURIFICATION:AffinityPurifiedPHYSICALSTATE:LiquidBUFFER:100uLin10mMHEPES(pH7.5),150mMNaCl,100ugpermLBSAand50%glycerol.STORAGECONDITIONS:5Lipoxygenaseantibodycanbestoredat-20˚Candisstableat-20˚Cforatleast1year.CLONALITY:PolyclonalCONJUGATE:UnconjugatedAdditionalInfoALTERNATENAMES:5-LO,5LPG,LOG5,5-LOX,ACCESSIONNO.:P09917PROTEINGINO.:126407OFFICIALSYMBOL:ALOX5GENEID:240BackgroundBACKGROUND:Theenzyme5-lipoxygenase(5-LO)playsakeyroleinregulatingtheproductionofleukotrienes(LTs)(Funk,2001).OverproductionofLTscontributestoseveraldiseases,mostnotablychronicinflammatorydiseases,includingasthma(Drazenetal.,1994),fibrosis(Wilbornetal.,1996)andatherosclerosis(Dwyeretal.,2004).Recentworkhasdemonstratedthattheactivityof5-LOisregulatedbyPKAphosphorylationofserine-523in5-LO(Luoetal.,2004).Undernormalconditions,thisphosphorylationmaybeimportantinlimitinginflammation.AbnormalsignalingthroughcAMPandPKA,then,couldcontributetoavarietyofdiseases,includingthosecharacterizedbychronicinflammation.Thephospho-specificantibodytoSer523on5-LOisthuslikelytoprovideavaluabletoolforstudiesoftheroleof5-LOregulationindiseasessuchasasthma,fibrosisandatherosclerosis.REFERENCES:1)DrazenJM,LillyCM,SperlingR,RubinP,IsraelE(1994)Roleofcysteinylleukotrienesinspontaneousasthmaticresponses.Adv.ProstaglandinThromboxaneLeukotRes22:251-262.2)DwyerJH,AllayeeH,DwyerKM,FanJ,WuH,MarR,LusisAJ,MehrABIanM(2004)Arachidonate5-lipoxygenasepromotergenotype,dietaryarachidonicacid,andatherosclerosis.NewEnglandJMed350:29-37.3)Funk,CD(2001)Prostaglandinsandleukotrienes:advancesineicosanoidBIOLOGy.Science294:1871-1875.