SpecificationsSPECIES:HumanSOURCESPECIES:E.coliSEQUENCE:MERTRSLQDPCSNCPAGTFCDNNRNQICSPCPPNSFSSAGGQRTCDICRQCKGVFRTRKECSSTSNAECDCTPGFHCLGAGCSMCEQDCKQGQELTKKGCKNCCFGTFNDQKRGICRPWTNCSLDGKSVLVNGTKERDVVCGPSPADLSPGASSVTPPAPAREPGHSTESTEDAPPLICATIONS:BIOLOGICALACTIVITY:Biologicalactivitywasdeterminedbyitsinhibitoryeffectofthe4-1BBLmediatedstimulationofIL-8productionbyhumanPBMC.About90%ofinibitionwasseenusingaconcentrationof1μgforboth4-1BBLand4-1BBR.PropertiesPURITY:Greaterthan98%bySDS-PAGEgelandHPLCanalyses.Endotoxinlevelislessthan0.1ngperμg(1EU/μg).PHYSICALSTATE:LyophilizedSTORAGECONDITIONS:Thelyophilized4-1BBReceptorrecombinantproteinisstableforatleast2yearsfromdateofreceiptat-20˚C.Reconstituted4-1BBReceptorisstableforatleast3monthswhenstoredinworkingaliquotswithacarrierproteinat-20˚C.Aswithanyprotein,exposing4-1BBReceptorrecombinantproteintorepeatedfreeze/thawcyclesisnotrecommended.Whenworkingwithproteinscareshouldbetakentokeeprecombinantproteinatacoolandstabletemperature.AdditionalInfoALTERNATENAMES:ILA,4-1BB,CD137,CDw137,ILA,Tumornecrosisfactorreceptorsuperfamilymember9,4-1BBligandreceptorACCESSIONNO.:NP_001552.2PROTEINGINO.:5730095Background4-1BBReceptor,amemberoftheTNFsuperfamilyofreceptors,ismainlyexpressedonthesurfaceofavarietyofTcells,butalsofoundinBcells,monocytes,andvarioustransformedcelllines.4-1BBReceptorbindsto4-1BBLtoprovideaco-stimulatorysignalforTlymphocytes.Signalingby4-1BBReceptorhasbeenimplicatedintheantigen-presentationprocessandgenerationofcytotoxicTcells.Thehuman4-1BBReceptorgenecodesfora255aminoacidtypeItransmembraneproteincontaininga17aminoacidN-terminalsignalsequence,a169aminoacidextracellulardomain,a27aminoacidtransmembranedomainanda42aminoacidcytoplasmicdomain.Recombinanthumansoluble4-1BBReceptorisa167aminoacidpolypeptide(17.7kDa),whichcontainsthecysteinerichTNFR-likeextracellulardomainof4-1BBReceptor.