产品说明
SpecificationsSPECIESREACTIVITY:Human,Mouse,RatTESTEDAPPLICATIONS:WBAPPLICATIONS:Westernblot:1-3ug/mlProvidedassayconcentrationsaresuggestionsonly,14-3-3sigmaantibodytitrationmayberequiredforoptimalresults.USERNOTE:OptimaldilutionsforeachapplicationtobedeterminedbytheresearcherIMMUNOGEN:Arecombinantproteinfragmentfromtheinternalregionof14-3-3sigmawasusedastheimmunogenforthisantibody.Theaminoacidsequenceiscommontothe~30kDaand~20kDaisoforms.HOSTSPECIES:RabbitPropertiesPURIFICATION:ProteinAaffinitychromatographyPHYSICALSTATE:LiquidBUFFER:PBSwith0.1mg/mlBSAand0.05%sodiumazideCONCENTRATION:0.5mg/mLSTORAGECONDITIONS:AliquotandStoreat-20C.Avoidfreez-thawcycles.CLONALITY:PolyclonalISOTYPE:IgGCONJUGATE:UnconjugatedAdditionalInfoALTERNATENAMES:14-3-3proteinsigma,EpithelialcellMarkerprotein1,Stratifin,SFN,HME1OFFICIALSYMBOL:SFNGENEID:2810BackgroundBACKGROUND:14-3-3proteinsareregulatorsofadiversegroupofsignalingproteins,includingkinases,phosphatases,andtransmembranereceptors,andhaverolesinawiderangeofphysiologicalprocessesincludingtranscription,intracellularsignaling,cellcyclecontrolandapoptosis.Thereareseven14-3-3proteinsinmostmammals:beta,gammaepsilon,zeta,eta,sigmaandtau.14-3-3sigma,alsocalledStratifin,isaproductoftheSFNgene.ItisatumorsuppressorgeneinactivatedviaDNAmethylation.Reducedornoexpressionof14-3-3sigmaisseeninvariouscarcinomaincludingskin,breastandprostate,suggestingthat14-3-3genesilencingmaybeinvolvedintumorprogression.
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