产品说明
SpecificationsSPECIESREACTIVITY:HumanHOMOLOGY:Predictedspeciesreactivitybasedonimmunogensequence:HamsterTESTEDAPPLICATIONS:IF,WBAPPLICATIONS:ForWBstartingdilutionis:1:1000ForIFstartingdilutionis:1:10~50USERNOTE:Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher.PREDICTEDMOLECULARWEIGHT:22kDaIMMUNOGEN:This27Kip1antibodyisgeneratedfromrabbitsimmunizedwithaKLHconjugatedsyntheticpeptidebetween165-194aminoacidsfromtheC-terminalregionofhuman27Kip1.HOSTSPECIES:RabbitPropertiesPURIFICATION:ThisantibodyispurifiedthroughaproteinAcolumn,followedbypeptideaffinitypurification.PHYSICALSTATE:LiquidBUFFER:SuppliedinPBSwith0.09%(W/V)sodiumazide.CONCENTRATION:0.42mg/mlSTORAGECONDITIONS:Storeat4˚Cforthreemonthsand-20˚C,stableforuptooneyear.Aswithallantibodiescareshouldbetakentoavoidrepeatedfreezethawcycles.Antibodiesshouldnotbeexposedtoprolongedhightemperatures.CLONALITY:PolyclonalISOTYPE:RabbitIgCONJUGATE:UnconjugatedAdditionalInfoALTERNATENAMES:Cyclin-dependentkinaseinhibitor1B,Cyclin-dependentkinaseinhibitorp27,p27Kip1,CDKN1B,KIP1ACCESSIONNO.:P46527OFFICIALSYMBOL:CDKN1BGENEID:1027BackgroundBACKGROUND:p27Kip1isacyclin-dependentkinaseinhibitor,whichsharesalimitedsimilaritywithCDKinhibitorCDKN1A/p21.TheencodedproteinbindstoandpreventstheactivationofcyclinE-CDK2orcyclinD-CDK4complexes,andthuscontrolsthecellcycleprogressionatG1.Thedegradationofthisprotein,whichistriggeredbyitsCDKdependentphosphorylationandsubsequentubiquitinationbySCFcomplexes,isrequiredforthecellulartransitionfromquiescencetotheproliferativestate.REFERENCES:1)Kawamata,N.,etal.,Eur.J.Haematol.74(5):424-429(2005).2)Andreu,E.J.,etal.,CancerRes.65(8):3264-3272(2005).3)Wingate,H.,etal.,J.Biol.Chem.280(15):15148-15157(2005).4)Wang,C.,etal.,J.Biol.Chem.280(13):12339-12343(2005).
ProSci
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