Specifications SPECIES REACTIVITY:Human TESTED APPLICATIONS:ELISA, WB APPLICATIONS:ELISA:Direct:To detect h4-1BBL by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant h4-1BBL.SandwichTo detect h4-1BBL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human 4-1BBL as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant h4-1BBL. Western Blot:To detect h4-1BBL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant h4-1BBL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. USER NOTE:Centrifuge vial prior to opening. IMMUNOGEN:Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant h4-1BBL (human 4-1BBLigand). Human 4-1BBL specific antibody was purified by affinity chromatography and then biotinylated. HOST SPECIES:Rabbit Properties PHYSICAL STATE:Lyophilized STORAGE CONDITIONS:4-1BBL antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. CLONALITY:Polyclonal CONJUGATE:Biotin Additional Info ALTERNATE NAMES:CD137L, 4-1BB-LTumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL ACCESSION NO.:P41273 PROTEIN GI NO.:728739 OFFICIAL SYMBOL:TNFSF9 GENE ID:8744 Background