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Encapsula/ImmunoFluor™-Glutaryl (Non-PEGylated)/2-ml/IMF-3155

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IMF-3155

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产品说明

Numerous techniques have been developed to prepare immunoliposomes based on the nucleophilic reactivity of free amine groups of proteins or peptides. However, the most common, versatile and straightforward activation chemistry for creating reactive acylating reagents and labeling peptides/proteins is to form NHS ester with primary amines. A single step nucleophilic substitution reaction between NHS ester derivative and alpha amines at the N terminal or the beta amines of lysine side chains leads to the formation of a stable amide bond.N-Hydroxysuccinimide (NHS) esters of DSPE-PEG-NHS liposomes react with the primary amine groups on the peptides, proteins, antibodies or other functional ligands for targeted drug delivery applications. The nucleophilic attack of the amine groups on the NHS-activated carbonyl group of the DSPE-PEG-NHS results in the elimination of the NHS group and formation of amide linkage.Conjugation reaction between NHS-activated DSPE-PEG lipid with the amine group of ligand and formation of stable amide linkage. The micelles formed from lipid conjugated ligand and non-reactive PEG lipids are mixed together and the PEGylated lipids are post-inserted into the liposomes to form PEGylated ligand surface conjugated liposomes.ImmunoFluor™-NHS is a PEGylated product. For other amine reactive (PEGylated and non-PEGyalated) products and also ImmunoFluor™ products suitable for other types of conjugation methods see here.