产品说明
Biotinylatedliposomescanbeconjugatednon-covalentlywith(strept)avidinthrougheitherdirectinteractionwiththeprotein/antibodyconjugatedto(strept)avidinorbycouplingwithotherbiotinylatedproteinsusing(strept)avidinasabridgingmolecule.Bothavidinand(strept)avidinformstrongnon-covalentbondwithbiotin.Thehighresistancetobreakdownmakesthemveryusefulinbioconjugatechemistry.However,(strept)avidinhasreplacedavidininmostbioconjugationapplicationsduetoitsenhancedproperties.NeutrAvidin(Thermofisher)isamodifiedavidinwithoutnegativeproperties.Itperformsmuchbetterthanoriginalavidinandsometimes(strept)avidin.Inordertoexploitthehigh-affinityinteractionofbiotinwith(strept)avidin,atwo-step“sandwich”protocol(MethodA)hasbeendevelopedforthepreparationoftargetedimmunoliposomes.Inthismethodology,(strept)avidinisfirstattachedtobiotinylatedliposomes,thenabiotin-modifiedprotein/antibodyisintroducedintothebiotinylated(strept)avidin-labeledliposomes.Thisnon-covalentapproachisrapid,extremelyversatileandapplicabletonumeroustargetingligandsofinterestwithrespecttoinvitroandinvivoapplications.Alternatively,insteadofforminga(strept)avidinbridge,(strept)avidinmoleculecanalsobecovalentlyconjugatedtoantibodyorligand(MethodB)andnon-covalentlyboundtoliposomescontainingbiotinonsurfaceinordertoformimmunoliposomes.ImmunoFluor™-BiotinisaNon-PEGylatedproduct.Forotherreactive(PEGylatedandnon-PEGyalatedproducts)biotinylatedliposomesandalsoImmunoFluor™productssuitableforothertypesconjugationmethodsseehere.
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