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BD Biosciences/CD3 FITC/CD19 PE/20 µL/349211
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产品说明
Product DetailsRecommended AssayReferencesDescriptionBecton Dickinson Immunocytometry Systems (BDIS) Simultest™ CD3/CD19 (Leu™-4/12) is a two-color direct immunofluorescence reagent for enumerating percentages of mature human T and B lymphocytes in erythrocyte-lysed whole blood (LWB).ContentsSpecificityCloneFormatIsotypeEntrez Gene IDCD3SK7 (also known as Leu-4)FITCIgG1, κ916CD194G7PEIgG1, κResources & ToolsSpectrumViewerDownload TDSDownload MSDSPreparation and Storage1. For in vitro diagnostic use.2. When stored at 2° to 8°C, the antibody reagent is stable until the expiration date shown on the label. Do not use after the expiration date.3. The antibody reagent should not be frozen or exposed to direct light during storage or during incubation with cells. Keep the reagent vial dry.4. Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, the reagent should not be used.5. The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.Antel J, Bania M, Noronha A, Neely S. Defective suppressor cell function mediated by T8+ cell lines from patients with progressive multiple sclerosis. J Immunol. 1986;137:3436-3439.Brenner M, Groh V, Porcelli A, et al. Plenary papers: Structure and distribution of the human T-cell receptor. In: Knapp W, Dörken B, Gilks W, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989:1049-1053.Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor-CD3 complex: A dynamic protein ensemble. Annual Rev Immunol. 1988;(6):629.Dörken B, Moller P, Pezzutto A, Schwartz-Albiez R, Molderhsuer G. B-cell antigens: CD19. In: Knapp W, Dörken B, Gilks W, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:34-36.Foucar K, Goeken J. Clinical application of immunologic techniques to the diagnosis of lymphoproliferative and immunodeficiency disorders. Lab Med. 1982;13:403-413.Giorgi J. Lymphocyte subset measurements: significance in clinical medicine. In: Rose N, Friedman H, Fahey J, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:236-246.Haynes B. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. New York, NY: Springer-Verlag; 1986;1:3-30.Jackson A, Warner N. Preparation, staining, and analysis by flow cytometry of peripheral blood leukocytes. In: Rose N, Friedman H, Fahey J, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:226-235.Kan E, Wang C, Wang L, Evans R. Non-covalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti-Leu-4 antibody. J Immunol. 1983;131:536-539.Knowles R. Immunochemical analysis of the T cell-specific antigens. In: Reinherz E, Haynes B, Nadler L, Bernstein I, eds. Leukocyte Typing II: Human T Lymphocytes. 1986:259-288.Ledbetter J, Evans R, Lipinski M, Cunningham-Rundles C, RA G, Herzenberg L. Evolutionary conservation of surface molecules that distinguish T-lymphocyte helper/inducer and T cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981;153:310-323.Nadler L. B cell/leukemia panel workshop: Summary and comments. In: Reinherz E, Haynes B, Nadler L, ID B, eds. Leukocyte Typing II: Human B Lymphocytes. New York, NY: Springer-Verlag; 1986:3–43.National Committee for Clinical Laboratory Standards. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes; Tentative Guideline (H42-T). 1992National Committee for Clinical Laboratory Standards. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture (H3-A3). 1991Ohno T, Kanoh T, Suzuki T, et al. Comparative analysis of lymphocyte phenotypes between carriers of human immunodeficiency virus (HIV) and adult patients with primary immunodeficiency using two-color immunofluorescence flow cytometry. J Exp Med. 1988;154:157.Prince H, Hirji K, Waldbeser L, Plaeger-Marshall S, Kleinman S, Lanier L. Influence of racial background on the distribution of T cell subsets and Leu-11-positive lymphocytes in healthy blood donors. Diag Immunol. 1985;3:33-37.Reichert T, DeBruyère M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopath. 1991;60:190-208.Schmidt R. Monoclonal antibodies for diagnosis of immunodeficiencies. Blut. 1989;59:200–206.Smolen J, Chused T, Leiserson W, Reeves J, Alling D, Steinberg A. Heterogeneity of immunoregulatory T-cell subsets in systemic lupus erythematosus. Am J Med. 1982;72:783-790.van Dongen J, Krissansen G, Wolvers-Tettero I, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988;71:603-612.
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