Product DetailsRecommended AssayReferencesDescriptionThe hsp90 family of molecular chaperones is involved in a number of signal transduction pathways, including cell division, protein folding and transport. A new member of this family was recently identified as TRAP1 (Tumor necrosis factor receptor-associated protein) or hsp75. TRAP1 is a mitochondrial protein that is an ATPase and ATP-binding protein, rendering it similar to hsp90. There is a high degree of homology between TRAP1 and hsp90 in 6 domains, however one major difference is that a highly charged domain present in hsp90 is absent in TRAP1. TRAP1 has been shown to interact with the intracellular domain of the type 1 receptor for tumor necrosis factor (TNFR-1IC) and to the retinoblastoma protein (Rb). Signaling through TNFR-1 induces antiviral responses, fibroblast proliferation, cytotoxicity, etc. TRAP1 binds TNFR-1IC outside of the death domain leading to disruption in the signaling by TNFR-1. Rb has been shown to play a pivotal role in cellular differentiation and progression through the cell cycle. TRAP1 associates with Rb via an LxCxE motif, which is common among proteins that interact with Rb through SV40 T-antigen binding domain. Thus, TRAP1 is a new member of the hsp90 family that interacts with Rb and TNFR-1IC. Format FormatPurifiedSuggested Companion ProductsHeLa Cell Lysate RUO 500µgCat No: 611449 HRP Goat Anti-Mouse Ig RUO 1mLCat No: 554002 Resources & ToolsSpectrumViewerDownload TDSRegulatory Document WebsitePreparation and StorageThe monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at -20°C.Product NoticesSince applications vary, each investigator should titrate the reagent to obtain optimal results.Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.Source of all serum proteins is from USDA inspected abattoirs located in the United States.Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml .Chen CF, Chen Y, Dai K, Chen PL, Riley DJ, Lee WH. A new member of the hsp90 family of molecular chaperones interacts with the retinoblastoma protein during mitosis and after heat shock. Mol Cell Biol. 1996; 16(9):4691-4699.View referenceFelts SJ, Owen BA, Nguyen P, Trepel J, Donner DB, Toft DO. The hsp90-related protein TRAP1 is a mitochondrial protein with distinct functional properties. J Biol Chem. 2000; 275(5):3305-3312.View referenceSong HY, Dunbar JD, Zhang YX, Guo D, Donner DB. Identification of a protein with homology to hsp90 that binds the type 1 tumor necrosis factor receptor. J Biol Chem. 1995; 270(8):3574-3581.View reference
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Rat Granulocytes Pure RP-1 500ug |
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550002 | BD | Ms Ig Kpa Lgt Chain FITC 187.1 500ug |
550007 | BD | ICC Fixation Buffer 100mL |
550011 | BD | Ms CD210 NALE 1B1.3a 500ug |
550019 | BD | Ms CD51 Pure RMV-7 100ug |
550026 | BD | Ms CD5 APC 53-7.3 100ug |
550037 | BD | Hu CD62E PE-Cy5 68-5H11 100Tst |
550041 | BD | Hu CD95 Pure EOS9.1 100ug |
550052 | BD | Ham IgG2 Kpa ItCl FITC B81-3 250ug |
550057 | BD | Ms Crry/p65 Pure 1F2 100ug |
550059 | BD | Ms IL-4 Recom Protein 10ug |
550068 | BD | Ms IL-2 Recom Protein 20ug |
550070 | BD | Hu IL-6 Recom Protein 10ug |
550072 | BD | Hu MIP-1 Bta PE D21-1351 100ug |
550082 | BD | Ms IgG1 PE A85-1 100ug |
550084 | BD | Ham IgG2 Kpa ItCl PE B81-3 100ug |
550086 | BD | Hu CD142 Pure HTF-1 100ug |
550254 | BD | Hu CD1d PE CD1d42 100Tst |
550256 | BD | Hu CD73 PE AD2 100Tst |
550258 | BD | Hu CD103 FITC Ber-ACT8 100Tst |
550260 | BD | Ms CD11c APC HL3 100ug |
550268 | BD | Ms CD72b/c Aloatg PE JY/93 100ug |
550270 | BD | Rat CD32 Pure D34-485 500ug |
550272 | BD | Rat CD32 NALE D34-485 500ug |
550274 | BD | Ms CD3e IHC Pure 145-2C11 1mL |
550277 | BD | Ms CD4 IHC Pure H129.19 1mL |
550280 | BD | Ms CD8a IHC Pure 53-6.7 1mL |