Product DetailsRecommended AssayReferencesDescriptionBD Simultest™ CD3/Anti–HLA-DR is a two-color direct immunofluorescence reagent for enumerating percentages of mature human activated T lymphocytes in erythrocyte-lysed whole blood (LWB).ContentsSpecificityCloneFormatIsotypeEntrez Gene IDCD3SK7 (also known as Leu-4)FITCIgG1, κ916HLA-DRL243PEIgG2a, κ0Resources & ToolsSpectrumViewerRegulatory Document WebsitePreparation and Storage• For in vitro diagnostic use.• When stored at 2° to 8°C, the antibody reagent is stable until the expiration date shown on the label. Do not use after the expiration date.• The antibody reagent should not be frozen or exposed to direct light during storage or during incubation with cells. Keep the reagent vial dry.• Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, the reagent should not be used.• The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Lymphocytes: Approved Guideline. Wayne, PA: Clinical and Laboratory Standards Institute; 1998. NCCLS document H42-A.Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture–Fourth Edition; Approved Standard. Wayne, PA: National Committee for Clinical Laboratory Standards; 1998. NCCLS document H3-A4.Protection of Laboratory Workers from Occupationally Acquired Infections–Second Edition; Approved Guideline. Villanova, PA: National Committee for Clinical Laboratory Standards; 2001. NCCLS document M29-A2.Bishop GA, Hall BM, Duggin GG, Horvath JS, Sheil AGR, Tiller DJ. Immunopathology of renal allograft rejection analyzed with monoclonal antibodies to mononuclear cell markers. Kidney Internat. 1986;29:708- 717.Bogner J, Matuschke B, Hienrich B, Schreiber M, Nerl C, Goebel F. Expansion of activated T lymphocytes (CD3+HLA-DR+) detectable in early stages of HIV-1 infection. Klin Wochenschr. 1990;68:393-396.Brenner MB, Groh V, Porcelli SA, et al. Structure and distribution of the human γ/δ T-cell receptor. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Osford University Press; 1989:1049-1053.Brodsky FM. A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes. Immunogenetics. 1984;19:179-194.Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annu Rev Immunol. 1988;6:629-662.Edwards JA, Durant BM, Jones DB, Evans PR, Smith JL. Differential expression of HLA class II antigens in fetal human spleen: relationship of HLADP, DQ, and DR to immunoglobulin expression. J Immunol. 1986;137:490-497.Giorgi JV. Lymphocyte subset measurements: significance in clinical medicine. In: Rose NR, Friedman H, Fahey JL, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:236-246.Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. New York, NY: Springer-Verlag; 1986;1:3-30.Jackson AL, Warner N. Preparation, staining, and analysis by flow cytometry of peripheral blood leukocytes. In: Rose N, Friedman H, Fahey J, ed. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:226-235.Kan EAR, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti–Leu-4 antibody. J Immunol. 1983;131:536-539.Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. New York, NY: Springer-Verlag; 1986;1:259-288.Lampson LA, Levy R. Two populations of Ia-like molecules on a human B cell line. J Immunol. 1980;125:293-299.Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T-lymphocyte helper/inducer and T cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981;153:310-323.Legendre CM, Schiffrin A, Weitzner G, Colle E, Guttman RD. Two-color flow cytometry analysis of activated T-lymphocyte subsets in type 1 diabetes mellitus. Diabetes. 1988;37:792-795.Levacher M, Tallet S, Dazza M, Dournon E, Rouveix B, Pocidalo J. T activation marker evaluation in ARC patients treated with AZT: comparison with CD4+ lymphocyte count in nonprogressors and progressors towards AIDS. Clin Exp Immunol. 1990;81:177-182.Prince H, Hirji K, Waldbeser L, Plaeger-Marshall S, Kleinman S, Lanier L. Influence of racial background on the distribution of T cell subsets and Leu-11-positive lymphocytes in healthy blood donors. Diag Immunol. 1985;3:33-37.Reichert T, DeBruyère M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopath. 1991;60:190-208.Stites D, Casavant C, McHugh T, et al. Flow cytometric analysis of lymphocyte phenotypes in AIDS using monoclonal antibodies and simultaneous dual immunofluorescence. Clin Immunol Immunopathol. 1986;38:161-177.Terstappen LWMM, Hollander Z, Meiners H, Loken MR. Quantitative comparison of myeloid antigens on five lineages of mature peripheral blood cells. J Leuk Biol. 1990;48:138-148.Tomkinson B, Wagner D, Nelson D, Sullivan J. Activated lymphocytes during acute Epstein-Barr virus infection. J Immunol. 1987;139:3802-3807.van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988;71:603-612.Vanham G, Kestens L, Gigase P, et al. Evidence for circulating activated cytotoxic T cells in HIVinfected subjects before the onset of opportunistic infections. Clin Exp Immunol. 1990;82:3-9.Warnke R, Levy R. Detection of T and B cell antigens hybridoma monoclonal antibodies: a biotin-avidin-horseradish peroxidase method. J Histochem Cytochem. 1980;28:771-776.
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