Product DetailsRecommended AssayReferencesDescriptionBD Simultest™ Leucogate™ (CD45/CD14) is a two-color direct immunofluorescence reagent for establishing an optimal lymphocyte gate for immunophenotyping of erythrocyte-lysed whole blood (LWB). BD Simultest Leucogate is for in vitro diagnostic use with BD in vitro diagnostic reagents. See the appropriate BD in vitro diagnostic package insert for additional information.ContentsSpecificityCloneFormatIsotypeEntrez Gene IDCD452D1FITCIgG1, κCD14MφP9(also known as MφP-9)PEIgG2b, κ929Resources & ToolsSpectrumViewerDownload TDSDownload MSDSPreparation and Storage• For in vitro diagnostic use.• When stored at 2° to 8°C, the antibody reagent is stable until the expiration date shown on the label. Do not use after the expiration date.• The antibody reagent should not be frozen or exposed to direct light during storage or during incubation with cells. Keep the reagent vial dry.• Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, the reagent should not be used.• The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes; Tentative Guideline. Villanova, PA: National Committee for Clinical Laboratory Standard; 1992. NCCLS document H42-T.Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture–Fourth Edition; Approved Standard. Wayne, PA: National Committee for Clinical Laboratory Standards; 1998. NCCLS document H3-A4.Bernard A, Boumsell L, Hill C. Joint report of the First International Workshop on Human Leucocyte Differentiation Antigens by the investigators of the participating laboratories: M2 protocol. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman S, eds. Leucocyte Typing. New York, NY: Springer-Verlag; 1984:82-108.Bernstein ID, Self S. Joint report of the myeloid section of the Second International Workshop on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human Myeloid and Hematopoietic Cells. New York, NY: Springer-Verlag; 1984:1-25.Beverley P. Production and use of monoclonal antibodies in transplantation immunology. In: Touraine J, Trager J, Betuel H, eds. Transplantation and Clinical Immunology XI. 1980:87-94.Cobbold S, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael A, ed. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press Inc; 1986:788-803.Dimitriu-Bona A, Burmester G, Waters S, Winchester R. Human mononuclear phagocyte differentiation antigens. I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. J Immunol. 1983;130:145-152.Giorgi J. Lymphocyte subset measurements: significance in clinical medicine. In: Rose N, Friedman H, Fahey J, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:236-246.Goyert S, Tesio L, Ashman L, et al. Report on the CD14 Cluster Workshop. In: Knapp W, Dörken B, Gilks WR, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989:789-794.Herrmann F, Komischke B, Odenwald E, Ludwig W. Use of monoclonal antibodies as a diagnostic tool in human leukemia. I. Acute myeloid leukemia and acute phase of chronic myeloid leukemia. Blut. 1983;47:157-163.Jackson A, Warner N. Preparation, staining, and analysis by flow cytometry of peripheral blood leukocytes. In: Rose N, Friedman H, Fahey J, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:226-235.Jackson A. Basic phenotyping of lymphocytes: selection and testing of reagents and interpretation of data. Clin Immunol Newslett. 1990;10:43-55.Jayaram Y, Hogg N. Surface expression of CD14 molecules on human neutrophils. In: Knapp W, Dörken B, Gilks WR, et al., eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:796-797.Loken M, Brosnan J, Bach B, Ault K. Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry. Cytometry. 1990;11:453-459.Rowe D, Beverley P. Characterisation of breast cancer infiltrates using monoclonal antibodies to human leucocyte antigens. Br J Cancer. 1984;49:149-159.Schwinzer R. Cluster report: CD45/CD45R. In: Knapp W, Dörken B, Gilks WR, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press Inc; 1989:628-634.
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Rat Granulocytes Pure RP-1 500ug |
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550002 | BD | Ms Ig Kpa Lgt Chain FITC 187.1 500ug |
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550280 | BD | Ms CD8a IHC Pure 53-6.7 1mL |
