Product DetailsRecommended AssayReferencesDescriptionThe CD45RA antibody, clone L48, is derived from hybridization of Sp2/0 mouse myeloma cells with spleen cells from BALB/c mice immunized with low-buoyant– density human lymphocytes.The CD45RO antibody, clone UCHL-1, is derived from hybridization of P3/NS-1/1- Ag4-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with an interleukin-2 (IL-2)–dependent human T-cell line.The CD3 antibody, clone SK7, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.The CD8 antibody, clone SK1, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.The CD45RA antibody recognizes a 220-kilodalton (kDa) molecular weight isoform of the leucocyte common antigen (LCA). The CD45RA antigen is a member of the CD45 antigen family that also includes the CD45, CD45RB, and CD45RO antigens.The CD45RO antibody, a member of the same CD45 family as CD45RA, recognizes a 180-kDa isoform of the LCA. The CD45 antigen is a protein tyrosine phosphatase.The CD3 antibody recognizes the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kDa. The antigen recognized by the CD3 antibody is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa).The CD8 antibody recognizes an antigen expressed on the 32-kDa α subunit of a disulfide-linked bimolecular complex. The cytoplasmic domain of the α subunit of the CD8 antigen is associated with the protein tyrosine kinase p56lck. The CD8 molecule interacts with class I major histocompatibility complex (MHC) molecules resulting in increased adhesion between the CD8+ T lymphocytes and the target cells. Binding of the CD8 molecule to class I MHC molecules enhances the activation of resting T lymphocytes.ContentsSpecificityCloneFormatIsotypeEntrez Gene IDCD8SK1APCIgG1, κ925CD45RAL48FITCIgG1, κ5788CD45ROUCHL1PEIgG2a, κ5788CD3SK7 (also known as Leu-4)PerCPIgG1, κ916Resources & ToolsSpectrumViewerDownload TDSDownload MSDSPreparation and StorageStore vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline — Third Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2005. CLSI document M29-A3.Akbar AN, Terry L, Timms A, Beverley PC, Janossy G. Loss of CD45R and gain of UCHL1 reactivity is a feature of primed T cells. J Immunol. 1988;140:2171-2178.Anderson P, Blue M-L, Morimoto C, Schlossman SF. 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