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BD Biosciences/Kit with BD Trucount™ Tubes/662415
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Product DetailsRecommended AssayReferencesDescriptionThe BD Leucocount™ kit consists of BD Leucocount™ reagent (propidium iodide fluorescent dye) and BD Trucount™ tubes and is intended for use with the BD FACSCalibur™, BD FACSort™, BD FACScan™, and BD FACSVia™ flow cytometer systems, or for a flow cytometer equipped with a 488-nm argon ion laser able to threshold on FL2, for enumerating residual white blood cells (rWBCs) in leucoreduced blood products.Resources & ToolsSpectrumViewerDownload MSDSPreparation and Storage• Store BD Leucocount reagent at 2°C– 8°C. Do not use after the expiration date shown on the label. • Avoid unnecessary exposure of the reagent to light.• Do not freeze the reagents or expose them to direct light during storage or incubation with cells. Keep the reagent vials dry.• Store BD Trucount tubes in their original foil pouch at 2°C–25°C. To avoid potential condensation, open the pouch only after it has reached room temperature and carefully reseal the pouch immediately after removing a tube. Examine the desiccant each time you open the pouch. If the desiccant has turned from blue to lavender, discard the remaining tubes. Use tubes within 1 hour after removal from the foil pouch. Once the pouch has been opened, the tubes are stable for 1 month. Do not use beyond the expiration date indicated on the packaging.Protection of Laboratory Workers fromOccupationally Acquired Infections; ApprovedGuideline—Third Edition. Wayne, PA: Clinical andLaboratory Standards Institute; 2005. CLSIdocument M29-A3.Bull BS, Breton-Corius J. Morphology of the erythron. In: Beutler E, Lichtman MA, Coller BS, Kripps TJ, eds. Williams Hematology. 5th ed. New York, NY: McGraw-Hill, Inc; 1995:349.Center for Biologics Evaluation and Research. Recommendations and Licensure Requirements for Leukocyte-Reduced Blood Products. Rockville, MD: Food and Drug Administration; 1996.Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.de Graan-Hentzen YCE, Gratama JW, Mudde GC, et al. Prevention of primary cytomegalovirus infection in patients with hematologic malignancies by intensive white cell depletion of blood products. Transfusion. 1989;29:757-760.Sniecinski I, O"Donnell MR, Nowicki B, Hill LR. Prevention of refractoriness and HLAalloimmunization using filtered blood products. Blood. 1988;71:1402-1407.Wenz B, Gurtlinger K, O"Toole A, Dugan E. Preparation of granulocyte-poor red blood cells by microaggregate filtration. A simplified method to minimize febrile transfusion reactions. Vox Sang. 1980;39:282-287.Dumont IJ, Dzik WH, Rebulla P, Brandwein H. Practical guidelines for process validation and process control of white cell-reduced blood components: report of the Biomedical Excellence for Safer Transfusion (BEST) Working Party of the International Society of Blood Transfusion (ISBT). Transfusion. 1996;36:11-20.Rebulla P, Porretti L, Bertolini F, et al. White cell-reduced red cells prepared by filtration: a critical evaluation of current filters and methods for counting residual white blood cells. Transfusion. 1993;33:128-133.Vanhula M, Simpson SJ, Martinson JA, et al. A flow cytometric method for counting very low levels of white cells in blood and blood components. Transfusion. 1993;33:262-267.Venglen-Tyler V, ed. Leukoreduction of RBC and platelet units. American Association of Blood Banks; 1996:722-725.

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