Product DetailsRecommended AssayReferencesDescriptionThe BD™ HLA-B27 system is a qualitative two-color direct immunofluorescence method for the rapid detection of HLA-B27 antigen expression in erythrocyte-lysed whole blood (LWB) using the BD FACSCanto™ family of flow cytometers, or the BD FACSCalibur™, BD FACSort™, or BD FACScan™ flow cytometer.Contents: Anti-HLA-B27 FITC / CD3 PE, HLA-B27 calibration beads, and BD FACS™ Lysing Solution.Resources & ToolsSpectrumViewerCFDA LicenseDownload TDSDownload MSDSPreparation and Storage• Store Reagent A and Reagent C upright at 2° to 8°C. Do not use after the expiration date shown on the label.• Store Reagent B, undiluted, at 2° to 25°C. When diluted, store at room temperature (20° to 25°C) for up to one month.• Do not freeze the reagents or expose them to direct light during storage or incubation with cells. Keep reagent vials dry.Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture–Fourth Edition; Approved Standard. Wayne, PA: National Committee for Clinical Laboratory Standards; 1998. NCCLS document H3-A4.Protection of Laboratory Workers from Occupationally Acquired Infections–Second Edition; Approved Guideline. Villanova, PA: National Committee for Clinical Laboratory Standards; 2001. NCCLS document M29-A2.Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.Dausset J. The major histocompatibility in man. Past, present, and future concepts. Science. 1981;213:1469-1474.Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. New York, NY: Springer-Verlag; 1986;1:3-30.Johnson J, Garbutt J, Nelson K. A monoclonal antibody specific for HLA-B27. Human Immunol. 1985;134-135.Kan EAR, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti–Leu-4 antibody. J Immunol. 1983;131:536-539.Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. New York, NY: Springer-Verlag; 1986;1:259-288.Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T-lymphocyte helper/inducer and T cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981;153:310-323.Levering WH, Wind H, Sintnicolaas K, Hooijkaas H, Gratama JW. Flow cytometric HLA-B27 screening: cross-reactivity patterns of commercially available anti-HLA-B27 monoclonal antibodies with other HLA-B antigens. Cytometry B Clin Cytom. 2003;54B:28-38.Lopez-Larrea C, Gonzalez-Roces S, Alvarez V. HLA-B27 structure, function, and disease association. Curr Opin Rheumatol. 1996;8:296-308.Müller H, Albrecht J, Schabel A. New aspects in HLA-typing by use of monoclonal antibodies and calibrated flow cytometry. Fresenius A Anal Chem. 1988;330:341.Seipp MT, Erali M, Wies RL, Wittwer C. HLA-B27 typing: evaluation of an allele-specific PCR melting assay and two flow cytometric antigen assays. Cytometry B Clin Cytom. 2005;63:10-15.Taurog JD, El-Zaatari FA. In vitro mutagenesis of HLA-B27. Substitution of an unpaired cysteine residue in the alpha 1 domain causes loss of antibody-defined epitopes. J Clin Invest. 1988;82:987-992.
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Rat Granulocytes Pure RP-1 500ug |
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550002 | BD | Ms Ig Kpa Lgt Chain FITC 187.1 500ug |
550007 | BD | ICC Fixation Buffer 100mL |
550011 | BD | Ms CD210 NALE 1B1.3a 500ug |
550019 | BD | Ms CD51 Pure RMV-7 100ug |
550026 | BD | Ms CD5 APC 53-7.3 100ug |
550037 | BD | Hu CD62E PE-Cy5 68-5H11 100Tst |
550041 | BD | Hu CD95 Pure EOS9.1 100ug |
550052 | BD | Ham IgG2 Kpa ItCl FITC B81-3 250ug |
550057 | BD | Ms Crry/p65 Pure 1F2 100ug |
550059 | BD | Ms IL-4 Recom Protein 10ug |
550068 | BD | Ms IL-2 Recom Protein 20ug |
550070 | BD | Hu IL-6 Recom Protein 10ug |
550072 | BD | Hu MIP-1 Bta PE D21-1351 100ug |
550082 | BD | Ms IgG1 PE A85-1 100ug |
550084 | BD | Ham IgG2 Kpa ItCl PE B81-3 100ug |
550086 | BD | Hu CD142 Pure HTF-1 100ug |
550254 | BD | Hu CD1d PE CD1d42 100Tst |
550256 | BD | Hu CD73 PE AD2 100Tst |
550258 | BD | Hu CD103 FITC Ber-ACT8 100Tst |
550260 | BD | Ms CD11c APC HL3 100ug |
550268 | BD | Ms CD72b/c Aloatg PE JY/93 100ug |
550270 | BD | Rat CD32 Pure D34-485 500ug |
550272 | BD | Rat CD32 NALE D34-485 500ug |
550274 | BD | Ms CD3e IHC Pure 145-2C11 1mL |
550277 | BD | Ms CD4 IHC Pure H129.19 1mL |
550280 | BD | Ms CD8a IHC Pure 53-6.7 1mL |
