Product DetailsRecommended AssayReferencesDescriptionThe BD FACSCount™ reagent kit is intended for in vitro diagnostic use in enumerating the absolute counts of CD4, CD8, and CD3 T lymphocytes in unlysed whole blood, using the BD FACSCount™ instrument.Resources & ToolsSpectrumViewerCFDA LicenseDownload MSDSDownload TDSPreparation and StorageEach antibody reagent is stable until the expiration date shown on the label when stored at 2°C to 8°C. Do not use after the expiration date. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the outside of the reagent vial dry.Do not use the reagent if you observe any change in appearance. Precipitation or discoloration indicates instability or deterioration.Protection of Laboratory Workers from Occupationally Acquired Infections–Third Edition; Approved Guideline. Wayne, PA: Clinical and Laboratory Standards Institute; 2005. M29-A3.Angadi CV. Lack of Leu-3a epitope on T-helper (CD4) lymphocytes. 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Induction of immunoglobulin-secreting cells in the allogeneic mixed leukocyte reaction: regulation by helper and suppressor lymphocyte subsets in man. J Immunol. 1981;127:931-935.Kurrle R. Cluster report: CD3. In: Knapp W, Dörken B, Gilks WR, et al., eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:290-293.Ledbetter JA, Evans RL, Lipinski M, CunninghamRundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981;153:310-323.Moebius U. Cluster report: CD8. In: Knapp W, Dörken B, Gilks WR, et al., eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:342-343.Reichert T, DeBruyère M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopath. 1991;60:190-208.Schmidt RE. Monoclonal antibodies for diagnosis of immunodeficiencies. Blut. 1989;59:200-206.Terry LA, DiSanto JP, Small TN, Flomenberg N. Differential expression of the CD8 and Lyt-3 antigens on a subset of human T-cell receptor γ/δ- bearing lymphocytes. In: Knapp W, Dörken B, Gilks WR, et al., eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:345-346.van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988;71:603-612.Wood GS, Warner NL, Warnke RA. Anti–Leu-3/T4 antibodies react with cells of monocyte/ macrophage and Langerhans lineage. J Immunol. 1983;131:212-216.