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BD Biosciences/CD4 Reagents/339010
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Product DetailsRecommended AssayReferencesDescriptionBD FACSCount™ CD4 reagents are used to enumerate the absolute counts of CD4 T lymphocytes and determine the percentage of lymphocytes that are CD4 T lymphocytes in unlysed whole blood (CD4 counts and CD4 percentages). The reagents are intended for in vitro diagnostic use on a BD FACSCount™ instrument.Contents: CD4 PE / CD14 PE-Cy™5 / CD15-PE-Cy5, fluorescent nuclear dye, and reference beads; reagent tube caps; fixative solution.Resources & ToolsSpectrumViewerDownload MSDSPreparation and StorageThe reagent is stable until the expiration date shown on the label when stored at 2°C to 8°C. Do not use after the expiration date. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the outside of the reagent vial dry.Do not use the reagent if you observe any change in appearance. Precipitation or discoloration indicates instability or deterioration.Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture; Approved Standard—Sixth Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2007. H3-A6.Protection of Laboratory Workers from Occupationally Acquired Infections–Third Edition. Villanova, PA: Clinical and Laboratory Standards Institute; 2005. CLSI document M29-A3.Bernard A, Boumsell L, Hill C. Joint report of the first international workshop on human leucocyte differentiation antigens by the investigators of the participating laboratories. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF, eds. Leucocyte Typing. New York, NY: Springer-Verlag; 1984:9-108.Bernstein ID, Self S. Joint report of the myeloid section of the Second International Workshop on Human Leukocyte Differentiation Antigens. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human Myeloid and Hematopoietic Cells. New York, NY: Springer-Verlag; 1986;3:1-25.Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.Dalgleish AG, Beverley PCL, Clapham PR, Crawford DH, Greaves MF, Weiss RA. The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature. 1984;312:763-767.Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man. J Exp Med.Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to the TH2 antigen. Proc Natl Acad Sci USA. 1981;78:544-548.Fahey JL, Taylor JM, Detels R, et al. The prognostic value of cellular and serologic markers in infection with human immunodefic. N Engl J Med. 1990;322:166-172.Flow Cytometry Checklist. In: College of American Pathologists (CAP) Flow Cytometry Checklist, Dec 2004:www.Cap.org/apps/docs/laboratory_accreditation/checklists_flow_cytometry_december2004.docGiorgi J. Lymphocyte subset measurements: significance in clinical medicine. In: Rose N, Friedman H, Fahey J, eds. Manual of Clinical Laboratory Immunology. 1986:236-246.Giorgi J. Lymphocyte subset measurements: significance in clinical medicine. In: Rose N, Friedman H, Fahey J, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:236-246.Goyert SM, Ferrero E. Biochemical analysis of myeloid antigen and cDNA expression of gp55 (CD14). In: McMichael AJ, ed. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1987:613-619.Kotzin BK, Benike CJ, Engleman EG. Induction of immunoglobulin secreting cells in the allogeneic mixed leukocyte reaction: regulation by helper and suppressor lymphocyte subsets in man. J Immunol. 1981;127:931-935.Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T-lymphocyte helper/inducer and T cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981;153:310-323.Lewis DE, Puck JM, Babcock GF, Rich RR. Disproportionate expansion of a minor T-cell subset in patients with lymphadenopathy syndrome and acquired immunodeficiency syndrome. J Infect Dis. 1985;151:555-559.Ohno T, Kanoh T, Suzuki T, et al. Comparative analysis of lymphocyte phenotypes between carriers of human immunodeficiency virus (HIV) and adult patients with primary immunodeficiency using two-color immunofluorescence flow cytometry. Tohoku J Exp Med. 1988;154:157-172.Reichert T, DeBruyère M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopath. 1991;60:190-208.Schmidt R. Monoclonal antibodies for diagnosis of immunodeficiencies. Blut. 1989;59:200–206.Skubitz K, Balke J, Ball E, et al. Report on the CD15 cluster workshop. In: Knapp W, Dörken B, Gilks W, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press Inc; 1989:800-805.Stites DP, Casavant CH, McHugh TM, et al. Flow cytometric analysis of lymphocyte phenotypes in AIDS using monoclonal antibodies and simultaneous dual immunofluorescence. Clin Immunol Immunopathol. 1986;38:161-177.
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