Product DetailsRecommended AssayReferencesDescriptionBD Procount™ progenitor cell enumeration kit is intended for use as an in vitro diagnostic test to identify and enumerate absolute counts and percentages of CD34+ cells in human peripheral blood samples, mobilized peripheral blood, and leukapheresis sample using flow cytometry.Contents: Combination of CD34 PE, CD45 PerCP, nucleic acid dye, and isotype controls; with BD Trucount™ tubes.Resources & ToolsSpectrumViewerDownload MSDSDownload TDSCFDA LicensePreparation and StorageIt is important that you carefully follow these instructions. Store the reagent vials upright at 2° to 8°C. Use opened vials within 4 weeks. Expiration dating for closed vials is shown on the label.Store BD Trucount tubes in theiroriginal foil pouch at 2° to 25°C. To avoid potential condensation, open the pouch only after it has reached room temperature andcarefully reseal the pouch immediately after removing a tube. Examine the desiccant each time you open the pouch. If the desiccant has turned from blue to lavender, discard the remaining tubes. Use tubes within 1 hour after removal from the foil pouch. Once the pouch has been opened, the tubes are stable for 1 month. Do not use beyond the expiration date indicated on the packaging.Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture; Approved Standard—Sixth Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2007. H3-A6.Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline—Third Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2005. CLSI document M29-A3.Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.Chen CH, Lin W, Shye S, et al. Automated enumeration of CD34+ cells in peripheral blood and bone marrow J Hematother. 1994;3:3-13.Cobbold SP, Hale G, Waldmann H. Non-lineage, LFA-1 family, and leucocyte common antigens: new and previously defined clusters. In: McMichael AJ, eds. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1987:788-803.Greaves MF, Titley I, Colman SM, et al. CD34 cluster workshop report. In: Schlossman SF, Boumsell L, Gilks W, et al, eds. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1995;1:840-846.Langenmayer I, Weaver C, Buckner CD, et al. Engraftment of patients with lymphoid malignancies transplanted with autologous bone marrow, peripheral blood stem cells or both. Bone Marrow Transplant. 1995;15:241-246.Lowdell MW, Bainbridge DR. External quality assurance for CD34 cell enumeration–results of a preliminary national trial. Royal Microscopical Society Clinical Flow Cytometry Group QA Schemes. Bone Marrow Transplant. 1996;17:849-853.Shpall EJ, Jones RB, Bearman SI, et al. Transplantation of enriched CD34-positive autologous marrow into breast cancer patients following high-dose chemotherapy: influence of CD34-positive peripheral-blood progenitors and growth factors on engraftment. J Clin Oncol. 1994;12:28-36.Zander AR, Lyding J, Bielack S. Transplantation with blood stem cells. Blood Cells. 1991;17:301- 309.
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