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Stemrd/VEGF-165, human recombinant/1000 µg/VEGF-1000

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VEGF-1000

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SourceHuman 293 CellsM.W.45kDaCAS No.Structural InfoFormulationLyophilized in sterile filtered solution of PBS.ReconstitutionBefore reconstitution, a brief spin is recommend to drive down any material dislodged from the bottom of the tube. The lyophilized protein should be reconstituted in sterile H2O to a desired concentration.StabilityThe lyophilized protein is stable for at least one year if stored at -80°C. Reconstituted protein is stable for at least four weeks at 4 °C, but should be stored in aliquots at -80 °C for longer term. Avoid repeated freeze and thaw.PurityGreater than 95% as determined by SDS-PAGE analysis and visualized by silver staining.Biological ActivityThe activity was determined by using a HUVEC cells. The ED 50 ranges from 2- 10 ng/ml. Activity in other assays should be determined by each individual setting. Country of OriginUSAVEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, it plays a prominent role in normal and pathological angiogenesis. StemRD’s VEGF-165 is made in human 293 cells as a 45kDa glycoprotein consisting of two 165 amino acid polypeptide chains.It is purified by aseries of chromatography. Catalog No. Size VEGF-01010µgVEGF-05050µgVEGF-250250 µgVEGF-10001000µgProduct Sheet

stemRD及其合作伙伴已经开发出一种高效的方法，可以在人细胞系中表达生物活性重组蛋白。由于这些细胞在无血清，无蛋白质和化学成分明确的培养基中生长，因此通过此过程制得的产品不含异种，并且具有人类特异性的转录后修饰，例如糖基化。使用该系统，我们已经能够生产出许多对干细胞的生长和分化至关重要的生物活性蛋白。