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Stemrd/Y-27632, ROCK inhibitor/25 mg/Y-025

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Y-025

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SourceM.W.320.26CAS No.146986-50-7Structural InfoFormulationPowderReconstitutionBefore reconstitution, we recommend abrief spin to drive down any material dislodged from the bottom of thetube. The compound is soluble in DMSO.StabilityThe powder is stable for at least 2year if stored at -20 degree C. Thedissolved compound is stable for at least 1 month at 4 degree C, but shouldbe stored in aliquots at -20 degree C for longer term. Protect from light.PurityGreater than 99% as determined by LC/MS analysis.LC/MS and/or NMR data available upon request.Biological ActivityTested active for culturing human iPS cells in serum-free medium on Matrigel coated plates. Country of OriginUSAY-27632, (1R,4r)-4-((R)-1-aminoethyl)-N-(pyridine-4-yl)cyclohexane-carboxamid-2HCl, is a Rho-associated kinase (ROCK) inhibitor. Y27632 has been shown to prevent apoptosisas well as enhance the survival and cloning efficiency of dissociated humanembryonic stem (ES) cells without affecting their pluripotency. Ref: Watanabe, K., etal. (2007). A ROCK inhibitor permits survival of dissociated human embryonicstem cells. Nat Biotechnol 25: 681-686.Product Sheet

stemRD及其合作伙伴已经开发出一种高效的方法，可以在人细胞系中表达生物活性重组蛋白。由于这些细胞在无血清，无蛋白质和化学成分明确的培养基中生长，因此通过此过程制得的产品不含异种，并且具有人类特异性的转录后修饰，例如糖基化。使用该系统，我们已经能够生产出许多对干细胞的生长和分化至关重要的生物活性蛋白。