首页 > Stemrd > Stemrd/WNT-5a, human/25 µg/W5A-H-025

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Stemrd/WNT-5a, human/25 µg/W5A-H-025

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W5A-H-025

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Stemrd

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SourceM.W.~45 kDa CAS No.Structural InfoFormulationLyophilized in sterile filtered solution of PBS with 1% CHAPSReconstitutionBefore reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of thisprotein, further dilutions should be made in buffer or medium containingcarrier proteins, such as albumin or serum.StabilityThe lyophilized protein isstable for at least 6 months if stored at -80 °C. Reconstituted protein is stable for at least 2weeks at 4 °C, but should be stored in aliquots at -80 °C forlonger term. Avoidrepeated freeze/thaw.PurityGreater than 85% as determined by SDS-PAGE and HPLC analysisBiological ActivityThe activity was determined by using a TCF reporter gene assay in 293 cells transfected with Frizzled-4 and LRP-5.WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay.Country of OriginUSAWNT-5a belongs to the class of WNT proteins thatactivate the “non-canonical” pathway. Thepredicted size of human WNT-5a is a monomeric protein containing 357 amino acidresidues. Due to glycosylation, itmigrates at an apparent molecular weight of ~45 kDa on SDS-PAGE undernon-reducing conditions. StemRD’sproduct is expressed from a human cell line in animal-free medium, and purifiedwith a proprietary process that is distinct from the published method.Product SheetYamada A, Iwata T, Yamato M et al., Diverse functionsof secreted frizzled-related proteins in the osteoblastogenesis of human multipotentmesenchymal stromalcells. Biomaterials. 2013 Apr;34(13):3270-8.http://www.ncbi.nlm.nih.gov/pubmed/23384792

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