SourceM.W.~40 kDa CAS No.Structural InfoFormulationLyophilized in sterile filtered solution of PBS with 1% CHAPSReconstitutionBefore reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of thisprotein, further dilutions should be made in buffer or medium containing carrierproteins, such as albumin or serum.StabilityThe lyophilized protein isstable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least 1month at 4 °C, but should be stored in aliquots at -80°C for longerterm. Avoid repeated freeze andthaw.PurityGreater than 90% as determined by SDS-PAGE analysisBiological ActivityThe activity was determined by using a TCF reporter gene assay in NIH3T3 cells.The EC50 ranges from 50 - 150 ng/ml. Country of OriginUSAWNT-3a is a member of the WNT family ofsignaling proteins that play a key role in embryonic development and theintegrity of adult tissues. WNT-3a is aprototypic canonical WNT that signals through the b-catenin pathway. The predicted size of mouse WNT-3a is amonomeric protein containing 333 amino acid residues. Due to glycosylation, it migrates at anapparent molecular weight of 40 kDa by SDS-PAGE analysis under non-reducingconditions. StemRD’s product isexpressed from a mouse cell line overexpressing mouse WNT-3a. Purification is performed with a proprietaryprocess that is distinct from the published method.Product SheetMae S, Shono A, Shiota F etal., Monitoring and robust induction of nephrogenic intermediatemesoderm from human pluripotent stem cells. Nature Communication. 2013;4:1367. http://www.ncbi.nlm.nih.gov/pubmed/23340407Chu, M. L. H., Ahn, V. E., Choi, H. J., et al., Structural Studies of Wnts andIdentification of an LRP6 Binding Site. Structure., 2013http://www.ncbi.nlm.nih.gov/pubmed/23791946