SourceM.W.38 - 41 kDa CAS No.Structural InfoFormulationLyophilized in sterile filtered solution of PBS with 1% CHAPSReconstitutionBefore reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of thisprotein, further dilutions should be made in buffer or medium containingcarrier proteins, such as albumin or serum.StabilityThe lyophilized protein isstable for at least 6 months if stored at -80 °C. Reconstituted protein is stable for at leasttwo weeks at 4 °C, but should be stored in aliquots at -80 °C forlonger term. Avoidrepeated freeze and thaw.PurityGreater than 90% as determined by SDS-PAGE and HPLC analysisBiological ActivityThe activity was determined by using a TCF reporter gene assay in cultured human cells.The EC50 ranges from 50 - 150 ng/ml. Country of OriginUSAWNT-3a is a member of the WNT family ofsignaling proteins that play key roles in embryonic development and the maintenanceof adult tissues. WNT-3a is a prototypiccanonical WNT that signals through the b-catenin pathway. The predicted size of human WNT-3a is amonomeric protein containing 328 amino acid residues. Due to glycosylation, it migrates at anapparent molecular weight of 38 - 41 kDa on SDS-PAGE under non-reducingconditions. StemRD’s WNT-3a is producedfrom a human cell line overexpressing human Wnt-3a cDNA in protein-free medium. Purification is done by a proprietary processthat is distinct from the published method. Product SheetKim SE, Huang H, Zhao M, et al., WntStabilization of β-Catenin Reveals Principles for Morphogen Receptor-ScaffoldAssemblies. Science. 2013 May 17; 340(6134):867-70http://www.ncbi.nlm.nih.gov/pubmed/23579495Zhang X, Abreu JG, Yokota C, et al., Tiki1 isrequired for head formation via Wnt cleavage-oxidation and inactivation. Cell. 2012 Jun 22;149(7):1565-77. http://www.ncbi.nlm.nih.gov/pubmed/22726442Hoshiba T, Kawazoe N, Chen G. Mechanism ofregulation of PPARG expressionof mesenchymal stem cells by osteogenesis-mimickingextracellular matrices. Biosci Biotechnol Biochem.2011;75(11):2099-104.http://www.ncbi.nlm.nih.gov/pubmed/22056426