SourceM.W.25 kDaCAS No.Structural InfoFormulationLyophilized in sterile filtered solution of acetonitrile/TFA and PBSReconstitutionBefore reconstitution, we recommend abrief spin to drive down any material dislodged from the bottom of thetube. The lyophilized protein shouldbe reconstituted in 5 mM HCl. Furtherdilutions should be made in buffer or medium containing carrier proteins,such as albumin or serum.StabilityThe lyophilized protein isstable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least 1month at 4°C, but should be stored in aliquots at -80 °C forlonger term. Avoidrepeated freeze and thaw.PurityGreater than 95% as determined by SDS-PAGE and HPLC analysesBiological ActivityThe activity was determined by using a SMAD reporter gene assay in human 293 cells. The EC50 ranges from 0.1 – 1.0 ng/ml. Country of OriginUSAOne of the three closely-related members of the TGF-β family. Human TGF-β1 cDNA encodes a 390 aa precursor with a 29 aa signal peptide. A furin-like convertase cleaves the precursor to form a 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-β1. Disulfide-linked mature TGF-beta1 is dissociated from the LAP upon activation. StemRD, produces active TGF-β1 in human 293 cells in Xeno-free medium. After a series of chromatography, the protein is purified to near homogeneity. Activity is determined by using a SMAD reporter gene assay with EC50 0.1 – 1.0 ng/mL.HPLCand SDS-PAGE profiles of TGF-β1, human from StemRD Inctgfb, tgf b, tgf beta Product SheetEyckmans J, Lin GL, Chen CS. Adhesive and mechanicalregulation of mesenchymal stem Cell differentiationin human bone marrow andperiosteum-derived progenitor cells. Biol Open.2012 Nov 15;1(11):1058-68.http://www.ncbi.nlm.nih.gov/pubmed/23213385