SourceHuman Mammalian CellsM.W.~45kDaCAS No.Structural InfoFormulationLyophilized in sterile filtered solution of PBS with 1% CHAPSReconstitutionBefore reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized protein shouldbe reconstituted in sterile H2O to a concentration of 100 ng/uL. Because of the hydrophobic nature of thisprotein, further dilutions should be made in buffer or medium containingcarrier proteins, such as albumin or serum.StabilityThe lyophilized protein isstable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least 1 month at 4 °C, but shouldbe stored in aliquots at -80 °C for longer term. Avoid repeated freeze andthaw.PurityGreater than 85% as determined by SDS-PAGE and HPLC analysisBiological ActivityThe activity was determined by using a Gli reporter gene assay in 3T3 cells.The EC50 ranges from 5 to 20 ng/ml. Country of OriginUSAThe hedgehog (Hh) gene encodes a secretedprotein originally identified in Drosophila. The vertebrate homologs of Hhcomprise of Sonic (SHh), Desert (DHh) and Indian (IHh). SHh plays an important role in embryonicdevelopment and in diseases such as cancer. Human SHh precursor is a45kDa protein. An autocatalytic reaction yields a 19 kDa N-terminaldomain that is biologically active. Because of unique post-translational modifications, SHh produced inmammalian cells is much more biologically active than SHh produced from E. coliand is devoid of artifact associated with the E. coli products commerciallyavailable from other manufacturers. SHh from StemRD made in human cells is 10-50 times more active, than other commercial products made in E. coli.Activity is detected by a Gli reporter gene assay in Swiss 3T3 cells.Product SheetChen Y, Yue S, Xie L, et al., DualPhosphorylation of suppressor of fused (Sufu) by PKA and GSK3beta regulates itsstability and localizationin the primary cilium. J Biol Chem. 2011 Apr 15;286(15):13502-11.http://www.ncbi.nlm.nih.gov/pubmed/21317289Ding H, Zhou D, Hao S, etal., Sonic hedgehog signaling mediates epithelial-mesenchymalcommunication and promotes renal fibrosis. JAm Soc Nephrol. 2012 May;23(5):801-13.http://www.ncbi.nlm.nih.gov/pubmed/22302193Wang J, Mook RA Jr, Lu J, et al., Identification of a novel Smoothenedantagonist that potently suppresses Hedgehog signaling. Bioorg Med Chem. 2012 Nov 15;20(22):6751-7. http://www.ncbi.nlm.nih.gov/pubmed/23063522Okabe S, Tauchi T, TanakaY, et al., Effects of the hedgehog inhibitor GDC-0449, alone or in combination with dasatinib, onBCR-ABL-positive leukemia cells. Stem Cells Dev. 2012 Nov1;21(16):2939-48.http://www.ncbi.nlm.nih.gov/pubmed/22642671