产品说明
SourceE. coliM.W.25 kDaCAS No.Structural InfoFormulationLyophilized from sterile filtered PBS solution, carrier-free.ReconstitutionBefore reconstitution, we recommend a brief spin to drivedown any material dislodged from the bottom of the tube. It is recommended to reconstitute the lyophilized product inacid solution such as 1mM HCl. Furtherdilutions should be made in sterile buffer or medium containing carrierproteins, such as albumin or serum.StabilityThe lyophilized protein is stable for at least 1 yearif stored at -80 °C. Reconstituted protein is stable for at least 2 week at 4 ° C, but shouldbe stored in aliquots at -80 °C for longer term. Avoidrepeated freeze/thaw.PurityGreater than 95% by SDS-PAGE analysisBiological ActivityThe activity was determined by proliferation of the NIH3T3 cells.The EC50 ranges from 1 – 5 ng/ml. Country of OriginUSAPlatelet-Derived Growth Factor (PDGF) is a mitogen for manycell types, such as fibroblast, smooth muscle cells and endothelial cells. StemRD produces PDGF-bb in Escherichia coli as a disulfide bond-linked homodimer with amolecular mass of 25 kDa. Thisproduct is suitable for tissue culture uses.Product Sheet
stemRD及其合作伙伴已经开发出一种高效的方法,可以在人细胞系中表达生物活性重组蛋白。由于这些细胞在无血清,无蛋白质和化学成分明确的培养基中生长,因此通过此过程制得的产品不含异种,并且具有人类特异性的转录后修饰,例如糖基化。使用该系统,我们已经能够生产出许多对干细胞的生长和分化至关重要的生物活性蛋白。
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