Taq polymerase5u/㎕, 50u/㎕Robust enzyme for routine PCR reactionsLow bacterial DNA contaminationHigh Yields & High SensitivityProductsCat.No.ProductFeatureSizeBT5Taq polymerase5u/㎕CustomBT50Taq polymerase50u/㎕CustomDescriptionDataSpecificationDocumentsTaq polymerase is a highly purified recombinant standard PCR enzyme. Host DNAs are removed almost entirely from the enzyme to minimize false positive reactions during molecular diagnostic applications. The 10x reaction buffer supplied together contains pH-buffering agent, salts, magnesium, and dNTPs. Application Routine PCR and RT-PCR Conventional and Real-Time PCRPCR for molecular diagnosticsManufacture of amplification mixtures  Figure 1. The purity of Taq polymerase 5u/㎕, 50u/㎕ (= HelixAmp™ Taq polymerase). The purity and concentration of the prepared enzyme were analyzed by SDS-PAGE with each units represented. St: Standard Protein marker.  Figure 2. Sensitivity of Taq polymerase 5u/㎕, 50u/㎕ (= HelixAmp™ Taq polymerase). Taq polymerase 5u/㎕, 50u/㎕ amplified target from genomic region of human PKD (polycystic kidney disease) gene at various concentrations of human genomic DNA. 1.25U of Taq DNA polymerases manufactured by NanoHelix and other companies were used.  Figure 3. Comparison of the PCR performances of commercial Taq polymerases on various sizes of targets. The PCRs were performed with 1.25 units of Taq polymerases and supplied buffers from each manufactures. All other components and conditions applied are identical through the reactions. Figure 4. Superior activity of Taq polymerase 5u/㎕, 50u/㎕ (= HelixAmp™ Taq polymerase). The PCRs were performed with indicated units of Taq polymerases and supplied buffers from each manufactures. All other components and conditions applied are identical through the reactions. NC, negative control reactions were performed with each 2.5 units of enzymes without template DNA. Enzyme activities: Highly processive 5"-3" DNA polymerase; double-strand specific 5"-3" exonuclease; no 3"-5" exonuclease activityTemperature optimum: Approximately +75°CStorage buffer: 20 mM Tris-HCl (pH 9.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Tween-20 and 50% (v/v) glycerol.Purity: ≥95% (SDS-PAGE)DNase contamination test: Not detectable (Incubation with 40U enzyme and pUC19 plasmid at 37°C, 1hr) RNase contamination test: Not detectable (Incubation with 40U enzyme and human total RNA at 37°C, 1hr)DNA contamination test: [E.coli DNA] less than one copy/5U enzyme, [Human DNA] Not detectableActivity test in PCR and qPCR: Correspond to reference (Human genomic DNA target)Stability: 24 months at -20°C.