Speed-Pfu PolymeraseHighest fidelity (15 ~ 20 times higher fidelity than Taq) Express speed. Fast PCR ( < 30 sec/kb extension ) Long-range PCR, up to 20kb from genomic DNA High productivityProductsCat.No.ProductSizeS250HelixAmp™ Speed-Pfu Polymerase250 unitsS500HelixAmp™ Speed-Pfu Polymerase500 unitsS2500HelixAmp™ Speed-Pfu Polymerase2,500 unitsS250NHelixAmp™ Speed-Pfu Polymerase (with dNTP Mix)250 unitsS500NHelixAmp™ Speed-Pfu Polymerase (with dNTP Mix)500 unitsS2500NHelixAmp™ Speed-Pfu Polymerase (with dNTP Mix)2,500 unitsDescriptionDataDetailsDocumentsHelixAmp™ Speed-Pfu polymerase is an editorial enzyme improved from HelixAmp™ Power-Pfu polymerase. HelixAmp™ Speed-Pfu polymerase shows faster (4~6 times) polymerization reaction and higher fidelity (3~5 times) than any other high-fidelity enzymes. With this high speed and accuracy, HelixAmp™ Speed-Pfu polymerase is most suitable to faithful amplification of relatively long-ranged target for cloning etc. Due to its high speed, fast PCR with this enzyme could be completed in 30 min for the reliable amplification of less than 1 kb size target DNA. For the maximum performance of PCR reactions high-quality dNTP mixture is supplied. The addition of TuneUp™ solution in the reaction mixture is a simple way to optimize the difficult targets of PCR.Figure 1. The express speed of HelixAmp™ Speed-Pfu polymerase. Four different sizes of human gene fragments were amplified with various extension times. PCR reactions were carried out at 95℃, 10 sec and 68℃ for the represented extension time (30 cycles). This result indicates that only 20-23 sec is required for successful synthesis of 1kb size DNA. The total PCR time required for amplification of 1 kb fragment is less than 25 minutes.  Figure 2. A fast PCR with HelixAmp™ Speed-Pfu polymerase and other enzymes. 1 kb fragment of human DNA was amplified from various concentrations of template in a limited extension time, 20 sec. PCR reaction was carried out at 95℃, 10 sec and 68℃, 20 sec (35 cycles).  Figure 3. HelixAmp™ Speed-Pfu polymerase is the enzyme with the highest fidelity. Error Rate = mutation/bp/duplication ApplicationHigh fidelity PCR Long range PCR Site-directed mutagenesis Blunt end PCR cloningContentsHelixAmp™ Speed-Pfu polymerase (2.5 units/㎕)10X Reaction bufferdNTP mix (each 10 mM)5X TuneUp™ solutionQuality ControlContamination assay for bacterial host DNA Activity assay Sensitivity assayStorage buffer50% Glycerol, 50mM Tris-HCl (pH8.0), 0.1mM EDTA, 1mM DTT, 0.1% Tween 20, 0.1% Nonidet P-40, 1mM PMSFManualMSDS