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NanoHelix/Ready-2x-MultiPlex (Ver. 2.0)/50 ml/MUUD01-M50
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产品说明
Ready-2x-MultiPlex (Ver. 2.0)Multiplex PCR up to 13-plex Allele specific multiplex PCR Automatic hot start PCR Extreme SpecificityProductsCat.No.ProductSizeMUD001SHelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) (with Dye) (1.25 ml x 2 tubes)2.5 mlMUD001LHelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) (with Dye) (1.25 ml x 10 tubes)12.5 mlMU001SHelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) (1.25 ml x 2 tubes)2.5 mlMU001LHelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) (1.25 ml x 10 tubeas)12.5 mlMUUD01-M5HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) [UDG System] (with Dye) (1 ml x 5 tubes)5 mlMUUD01-M50HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) [UDG System] (with Dye) (1 ml x 50 tubes)50 mlDescriptionDataDetailsDocumentsHelixAmp™ Ready-2x-MultiPlex (Ver. 2.0), the best choice to setup complex multiplexing PCR, is a pre-mixed solution containing HelixAmp™ Hot Taq DNA polymerase, dNTPs and optimized buffer as 2x concentration. HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0)is designed for hot-start PCR to allow the highest specificity and minimize the interruptions among the primers in a reaction mixture. This pre-mixed enzyme is highly suitable for up to 13-plex PCR, allele specific PCR (discriminating SNP), and applications for genotyping. The provided TuneUp™ solution helps to improve the yield and specificity in problematic PCRs. HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0)provides the most suitable condition for efficient and reproducible multiplex or specific PCR. Figure 1. Multiplex PCR of up to 8 targets using HelixAmp™ Ready-2x-MultiPlex. Multiplex PCR in single-tube reaction is performed with rice genomic DNA and mixture of allele-specific primer set corresponding to SNP. Primer set corresponding to each target was added into one-tube multiplex PCR one by one. M: Standard size marker Figure 2. Multiplex PCRs for various multiplex primer sets using HelixAmp™ Ready-2x-MultiPlex. Q : QIAGEN multiplex PCR premix, N : NanoHelix’s HelixAmp™ Ready-2x-MultiPlex. ApplicationMultiplex PCR Allele specific PCR ContentsHelixAmp™ Ready-2x-MultiPlex PCR Master Mix (Ver. 2.0)containing HelixAmp™ Hot Taq DNA polymerase (Ver. 2.0), Buffer, dNTPs and stabilizing agents. Quality ControlHelixAmp™ Ready-2x-Go series is evaluated by amplification compare with mixture of each component required in PCR for various size of target without further optimization at a different concentration of template DNA.ManualMSDSBrochure
NanoHelix热逆转录酶是M-MLV逆转录酶(RTase)的一种热稳定且RNase H阴性的变体,是一种可在42℃〜55℃的温度下从RNA模板合成cDNA的酶,在50℃时显示最高的活性。热逆转录酶的高生产力和生产力可以扩增产物的高产率,并且可以从RNA模板合成高达12 kb的靶基因cDNA。
应用
生成长达12 kb的第一链cDNA用于文库或克隆
两步或一步RT-PCR应用
常规或实时RT-PCR
RT-PCR检测病毒RNA
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