产品说明
Genomic DNA Prep Kit, Solution TypeHigh yield, minimum fragmentation Simple salting out method Easy scale-upSafe and economic ProductsCat.No.ProductSizeGSA100PureHelix™ Genomic DNA Prep Kit [Animal], Solution Type100 prepsGSA400PureHelix™ Genomic DNA Prep Kit [Animal], Solution Type400 prepsGSBA-100PureHelix™ Genomic DNA Prep Kit [Bacteria], Solution Type100 prepsGSBA-400PureHelix™ Genomic DNA Prep Kit [Bacteria], Solution Type400 prepsGSB100PureHelix™ Genomic DNA Prep Kit [Blood], Solution Type100 prepsGSB400PureHelix™ Genomic DNA Prep Kit [Blood], Solution Type400 prepsGSP100PureHelix™ Genomic DNA Prep Kit [Plant], Solution Type100 prepsGSP400PureHelix™ Genomic DNA Prep Kit [Plant], Solution Type400 prepsGSY100PureHelix™ Genomic DNA Prep Kit [Yeast], Solution Type100 prepsGSY400PureHelix™ Genomic DNA Prep Kit [Yeast], Solution Type400 prepsDescriptionDataDetailsDocumentsPureHelix™ Genomic DNA Prep Kit (Solution type) is designed for high-yield and high-quality isolation of genomic DNA from wide variety of starting materials including whole bloods, cultured cells, animal tissues, plant tissues, yeast, and bacteria. This solution-based system, simple and gentle salting out method, allows easy scale-up and minimizes the DNA fragmentation. Due to no use of phenol, this kit is safe and does not produce any harmful waste. DNA purified with this kit is suitable for a variety of applications, including PCR amplification, digestion with restriction endonucleases and membrane hybridizations. Figure 1. Isolation of genomic DNA from various samples using PureHelix™ Genomic DNA Prep Kit (Solution Type). The sample corresponding to animal, plant, bacteria and yeast uses the porcine muscle tissue, soybean leaf tissue, E.coli and S,cerevisiae respectively.Application PCR applicationSouthern blotting analysis. Cloning Genotyping Discovery and validation of biological markers (SNP or SSR) ManualMSDSBrochure
NanoHelix热逆转录酶是M-MLV逆转录酶(RTase)的一种热稳定且RNase H阴性的变体,是一种可在42℃〜55℃的温度下从RNA模板合成cDNA的酶,在50℃时显示最高的活性。热逆转录酶的高生产力和生产力可以扩增产物的高产率,并且可以从RNA模板合成高达12 kb的靶基因cDNA。
应用
生成长达12 kb的第一链cDNA用于文库或克隆
两步或一步RT-PCR应用
常规或实时RT-PCR
RT-PCR检测病毒RNA
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